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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1990-11-15
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pubmed:abstractText |
Using a cornea trigeminal ganglion model, we have investigated transcription by herpes simplex virus type 2 (HSV-2) during latency in mice. Latency was verified 2 months postinoculation by reactivation of HSV-2 after explant cocultivation of trigeminal ganglia from the majority of mice (83%). Transcription during latent HSV-2 infection was limited to the repeat regions of the viral genome as determined by in situ hybridization using restriction fragment probes representing 100% of the HSV-2 genome. Further mapping of the positively hybridizing region by using subfragments showed that transcription occurred from approximately 11.5 kb of contiguous DNA fragments. A 1.0-kb PvuI-BamHI fragment within the BamHI F fragment and a 0.3-kb BamHI-SalI fragment and a 3.4-kb SalI-BamHI fragment within the BamHI P fragment hybridized more strongly than other subfragments in in situ hybridization experiments. All positive signals were confined to the nucleus. The RNA that hybridized to the 3.4-kb SalI-BamHI DNA fragment probe by in situ hybridization corresponded to a 2.3-kb transcript on Northern (RNA) blots. Under our conditions for Northern blot hybridization, the 3.4-kb SalI-BamHI probe of HSV-2 hybridized to a limited degree with the latency-associated transcripts of HSV-1. Shorter spliced species of latency-associated transcript RNA, which are seen during HSV-1 latency, have not been detected in latent HSV-2 RNA. However, viral gene expression during HSV-2 latency appears to be very similar to that during HSV-1 latency.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-195220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2152989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2154530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2157803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-220797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2434993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2470921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2536115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2542019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2542601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2550571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2592985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2824817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2824818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2828516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2828517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2828670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2831380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2832064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2832852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2839575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2841498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2842950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2845123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2846760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2846893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2849116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-2991558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-3033640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-4333719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-4352382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-4371760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-6281477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-6284019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-6286797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2170675-6297753
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5342-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2170675-Animals,
pubmed-meshheading:2170675-Blotting, Northern,
pubmed-meshheading:2170675-Culture Techniques,
pubmed-meshheading:2170675-DNA, Viral,
pubmed-meshheading:2170675-DNA Probes,
pubmed-meshheading:2170675-Gene Expression Regulation, Viral,
pubmed-meshheading:2170675-Mice,
pubmed-meshheading:2170675-Nucleic Acid Hybridization,
pubmed-meshheading:2170675-RNA, Viral,
pubmed-meshheading:2170675-Restriction Mapping,
pubmed-meshheading:2170675-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2170675-Simplexvirus,
pubmed-meshheading:2170675-Transcription, Genetic,
pubmed-meshheading:2170675-Trigeminal Ganglion
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pubmed:year |
1990
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pubmed:articleTitle |
Characterization of herpes simplex virus type 2 transcription during latent infection of mouse trigeminal ganglia.
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pubmed:affiliation |
Wistar Institute, Philadelphia, Pennsylvania 19104-4268.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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