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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2011-6-23
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pubmed:abstractText |
Despite remarkable progress in the development of both viral and nonviral gene delivery vectors for airway disease treatment, poor gene transfer efficiency to the airway epithelium is a major obstacle in clinical application. To take advantage of the unique features of viral and nonviral vectors, we have developed complexes of adenovirus vector and anionic liposomes (AL-Ad5) by the calcium-induced phase change method. In the current study, based on the fact that there are overexpressed folate receptors on the surface of airway epithelia, we further modified the AL-Ad5 complexes with folate (F-AL-Ad5) to improve the transduction ability of Ad5 in airway epithelia. The transduction efficiencies of the obtained F-AL-Ad5 and AL-Ad5 complexes were assessed in primary-cultured airway epithelia in vitro. Our results indicated that compared with naked adenovirus vector, both AL-Ad5 and F-AL-Ad5 could significantly enhance the gene transduction efficiency of adenovirus vector in primary-cultured airway epithelial cells. Moreover, the enhancement mediated by F-AL-Ad5 was more dramatic than that by AL-Ad5. These results suggested that F-AL-Ad5 may be a useful strategy to deliver therapeutic genes to the airway epithelia and is promising in clinical application.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1178-2013
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1083-93
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pubmed:meshHeading |
pubmed-meshheading:21698075-Adenoviridae,
pubmed-meshheading:21698075-Analysis of Variance,
pubmed-meshheading:21698075-Animals,
pubmed-meshheading:21698075-Anions,
pubmed-meshheading:21698075-Edetic Acid,
pubmed-meshheading:21698075-Flow Cytometry,
pubmed-meshheading:21698075-Folic Acid,
pubmed-meshheading:21698075-Gene Transfer Techniques,
pubmed-meshheading:21698075-Genetic Vectors,
pubmed-meshheading:21698075-Lac Operon,
pubmed-meshheading:21698075-Liposomes,
pubmed-meshheading:21698075-Male,
pubmed-meshheading:21698075-Mice,
pubmed-meshheading:21698075-Mice, Inbred C57BL,
pubmed-meshheading:21698075-Particle Size,
pubmed-meshheading:21698075-Respiratory Mucosa
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pubmed:year |
2011
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pubmed:articleTitle |
Improvement of adenoviral vector-mediated gene transfer to airway epithelia by folate-modified anionic liposomes.
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pubmed:affiliation |
Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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