Linked Life Data

21692558

Source:http://linkedlifedata.com/resource/pubmed/id/21692558

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-6-22
pubmed:abstractText
Cytotoxic T lymphocyte (CTL) epitopes in the X protein (HBx) of hepatitis B virus (HBV) may play a key role in the viral control and liver damage. The aim of this study was to identify and study the function of HLA-A0201 restricted CTL epitopes in HBx of HBV genotypes B and C that are epidemic in China. Four nonapeptides signed HBx1: VLCLRPVGA, HBx2: CLFKDWEEL, HBx3: VLHKRTLGL, and HBx4: HLSLRGLPV were predicated by computational analysis and manually confirmed by defining the peptide supermotif, extended motif, and quantitative motif. Synthesized peptides were examined for their affinity and binding stability with HLA-A0201. After being analyzed by enzyme-linked immunospot (ELISPOT) and cytolytic activity assays, the HBx2 epitope was selected for a construction of HLA-A0201-peptide tetramers. The tetramer staining method was used to analyze peripheral blood mononuclear cells (PBMCs) isolated from HBV-infected patients at different disease stages (chronic hepatitis, liver cirrhosis, and hepatoma). Compared with CTL epitopes in the HBV envelope or polymerase, HBx2 is also a potential HLA-A0201 restricted CTL epitope, what may have a clinical implication.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-15
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Identification and functional studies of HLA-A0201 restricted CTL epitopes in the X protein of hepatitis B virus.
pubmed:affiliation
Department of Medical Genetics, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, P.R. China. shsun@vip.sina.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't