Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-9-21
|
| pubmed:abstractText |
Two monoclonal antibodies (mAbs) directed toward brain natriuretic peptide (BNP), KY-BNP-I and KY-BNP-II, have been produced. Both mAbs against BNP possessed high affinities for BNP, with association constants (Ka) of 4.0 X 10(9) M-1 (KY-BNP-I) and 2.0 X 10(10) M-1 (KY-BNP-II). With these mAbs, specific RIAs for BNP have been established. The least detectable quantities of BNP were 5 pg/tube (KY-BNP-I) and 1 pg/tube (KY-BNP-II). Cross-reactivities of alpha-human and rat atrial natriuretic polypeptides were less than 0.001%. These RIAs detected BNP-like immunoreactivity (BNP-LI) not only in the porcine brain but also in the canine brain, with the highest concentration in the medulla oblongata. These RIAs also detected BNP-LI in both the porcine and canine hearts and in the porcine plasma. The iv pretreatment of purified mAb[KY-BNP-II] almost completely blocked the hypotensive action of iv administered BNP in rats, with the concomitant suppression of BNP-induced increase of the plasma cyclic GMP level. These results indicate that our mAbs against BNP will serve as a useful tool for the elucidation of the physiological and pathophysiological significance of BNP as a neuropeptide and as a hormone.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
127
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
1292-300
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2167214-Animals,
pubmed-meshheading:2167214-Antibodies, Monoclonal,
pubmed-meshheading:2167214-Antigens,
pubmed-meshheading:2167214-Atrial Natriuretic Factor,
pubmed-meshheading:2167214-Blood Pressure,
pubmed-meshheading:2167214-Brain Chemistry,
pubmed-meshheading:2167214-Cyclic GMP,
pubmed-meshheading:2167214-Dogs,
pubmed-meshheading:2167214-Female,
pubmed-meshheading:2167214-Haplorhini,
pubmed-meshheading:2167214-Heart Atria,
pubmed-meshheading:2167214-Humans,
pubmed-meshheading:2167214-Hybridomas,
pubmed-meshheading:2167214-Immunization, Passive,
pubmed-meshheading:2167214-Male,
pubmed-meshheading:2167214-Mice,
pubmed-meshheading:2167214-Mice, Inbred BALB C,
pubmed-meshheading:2167214-Radioimmunoassay,
pubmed-meshheading:2167214-Rats,
pubmed-meshheading:2167214-Swine,
pubmed-meshheading:2167214-Tissue Distribution
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Preparation of monoclonal antibodies against brain natriuretic peptide and their application to radioimmunoassay and passive immunization.
|
| pubmed:affiliation |
Department of Medicine, Kyoto University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|