Linked Life Data

21660701

Source:http://linkedlifedata.com/resource/pubmed/id/21660701

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-6-10
pubmed:abstractText
Homologous recombination (HR) is a mode of double-strand break (DSB) repair required for cell viability in vertebrate cells. Targeted integration of homologous DNA fragment by HR is usually a very rare event in vertebrate cells; however, in chicken B lymphoma cell line DT40, the ratio of targeted to random integration is extremely high. Although the underlying mechanism of this phenotype is not fully understood, DT40 has been utilized as a model cell line for a number of genetic analyses. Here we describe three assays for evaluating homologous recombinational repair (HRR) using DT40 as a model system, measuring gene-targeting frequency, analyzing HRR process of single DSB induced by yeast homing endonuclease I-SceI, and measuring sister chromatid exchange frequency. Combined with generation of gene-disrupted DT40 mutant cell line, these assays have been highly useful to investigate the mechanisms in HRR. Using these techniques, a role of HRR of not only Rad52 epistasis group genes but also genes whose mutation cause hereditary cancer syndrome, such as Fanconi anemia, has been established.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
745
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-309
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Evaluation of homologous recombinational repair in chicken B lymphoma cell line, DT40.
pubmed:affiliation
Department of Molecular Oncology, Kyushu University, Kyushu, Japan. hkitao@surg2.med.kyushu-u.ac.jp
pubmed:publicationType
Journal Article