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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1990-9-11
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pubmed:abstractText |
The receptor for the neuropeptide gastrin-releasing peptide, the mammalian homologue of bombesin, was solubilized from rat brain and Swiss 3T3 cells by using the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS) and the cholesteryl hemisuccinate ester (CHS). Only the combination of the detergent CHAPS and the cholesteryl ester CHS in a glycerol-containing buffer satisfactorily preserved the binding activity upon solubilization. Specific binding activity was only solubilized from cell lines and tissue preparations known to express the GRP receptor. The dissociation constant (Kd) for the receptor solubilized from rat brain and Swiss 3T3 cells was 0.6 nM, similar to the value of 0.8 nM calculated for the membrane-bound receptor. Binding was saturable and reached equilibrium after approximately 2 h at 4 degrees C. The identity of the solubilized receptor with the membrane-bound one was further confirmed by the concordance of the relative binding affinities of various established bombesin analogues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5153-60
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2165804-Animals,
pubmed-meshheading:2165804-Binding Sites,
pubmed-meshheading:2165804-Bombesin,
pubmed-meshheading:2165804-Brain Chemistry,
pubmed-meshheading:2165804-Cell Line,
pubmed-meshheading:2165804-Cell Membrane,
pubmed-meshheading:2165804-Cholesterol Esters,
pubmed-meshheading:2165804-Cholic Acids,
pubmed-meshheading:2165804-Ligands,
pubmed-meshheading:2165804-Male,
pubmed-meshheading:2165804-Mice,
pubmed-meshheading:2165804-Rats,
pubmed-meshheading:2165804-Rats, Inbred Strains,
pubmed-meshheading:2165804-Receptors, Bombesin,
pubmed-meshheading:2165804-Receptors, Neurotransmitter,
pubmed-meshheading:2165804-Solubility
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pubmed:year |
1990
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pubmed:articleTitle |
Solubilization of the receptor for the neuropeptide gastrin-releasing peptide (bombesin) with functional ligand binding properties.
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pubmed:affiliation |
Rorer Biotechnology, Inc., King of Prussia, Pennsylvania 19406.
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pubmed:publicationType |
Journal Article
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