Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
22
|
| pubmed:dateCreated |
1990-9-4
|
| pubmed:abstractText |
The cloned long terminal repeats (LTRs) of mouse intracisternal A-particle (IAP) proviral elements differ in their promoter activity. In this study, the LTR from a recently transposed IAP element (rc-mos) is shown to be a more effective promoter both in vivo and in vitro than the LTR from a randomly cloned genomic element (MIA14). These LTRs differ in nucleotide sequence in certain previously defined protein-binding domains. In particular, the MIA14 LTR contains two domains, designated Enh1 and Enh2, with sequence homology to the SV40 enhancer core motif, while in rc-mos the Enh2 position is occupied by a variant sequence which lacks core homology. EBP-80 is a general enhancer core-binding protein originally isolated by virtue of its affinity for the MIA14 Enh2 sequence (Falzon, M., and Kuff, E.L. (1989) J. Biol. Chem. 264, 21915-21922). We now find by quantitative binding studies, binding competition, and UV cross-linking that EBP-80 from both human and mouse cells binds to the "Enh2" motif of rc-mos more strongly than to the Enh2 of MIA14. In vitro transcription from both LTRs is strongly enhanced by addition of EBP-80 showing that binding is related to function. The rc-mos LTR remains the more effective promoter in the presence of added EBP-80. Reciprocal substitution of the Enh2 domains in the two LTRs by site-directed mutagenesis shows that the rc-mos variant confers a 3-fold increment in in vivo promotor activity. The rc-mos motif or a closely related sequence is found in the cloned LTRs of many expressed and/or recently transposed IAP elements. EBP-80 is identified as a cellular transcription factor whose heightened levels in certain mouse cells might result in preferential expression of IAP elements containing this sequence motif.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
265
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
13084-90
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2165492-Animals,
pubmed-meshheading:2165492-Base Sequence,
pubmed-meshheading:2165492-Cell Line,
pubmed-meshheading:2165492-Cell Nucleus,
pubmed-meshheading:2165492-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2165492-Cloning, Molecular,
pubmed-meshheading:2165492-DNA Transposable Elements,
pubmed-meshheading:2165492-Genetic Variation,
pubmed-meshheading:2165492-HeLa Cells,
pubmed-meshheading:2165492-Humans,
pubmed-meshheading:2165492-Kinetics,
pubmed-meshheading:2165492-Mice,
pubmed-meshheading:2165492-Molecular Sequence Data,
pubmed-meshheading:2165492-Oligonucleotide Probes,
pubmed-meshheading:2165492-Plasmids,
pubmed-meshheading:2165492-Promoter Regions, Genetic,
pubmed-meshheading:2165492-Proviruses,
pubmed-meshheading:2165492-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2165492-Retroviridae,
pubmed-meshheading:2165492-Transcription, Genetic,
pubmed-meshheading:2165492-Transcription Factors
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
A variant binding sequence for transcription factor EBP-80 confers increased promoter activity on a retroviral long terminal repeat.
|
| pubmed:affiliation |
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
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| pubmed:publicationType |
Journal Article
|