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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1990-8-23
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pubmed:abstractText |
We have compared the in-vitro interactions of quinine and cinchonine, two alkaloids from cinchona bark, with human neutrophil functions. Although these molecules are structurally similar, they induced a quantitatively different depressive effect on neutrophil chemotaxis and oxidative response. Quinine produced the strongest effect at concentrations as low as 10 mg/l, which may be achievable in serum during therapeutic use of this compound. The depression induced by cinchonine was noticeable only at 100 mg/l. Chemotaxis was decreased by about 25% (formyl-methionyl-leucyl-phenylalanine) or 39% (serum) for quinine (100 mg/l) only if a constant concentration of the drug was maintained during the assay while cinchonine had no effect on this PMN function. The greatest impairment was observed for the PMN oxidative burst: this was dose-dependent whatever the stimulus used (phorbol-myristate-acetate or opsonized zymosan). After 30 min incubation in the presence of the drugs, the zymosan-induced chemiluminescence response was decreased by 96% and by 67% with quinine, 100 and 10 mg/l, respectively, and by 62% with cinchonine 100 mg/l. The myeloperoxidase-mediated iodination of PMN was reduced by 100% and 46% with quinine, 100 and 10 mg/l, respectively, whereas cinchonine decreased this function by about 95% at 100 mg/l and 14% at 10 mg/l. Superoxide anion generation was impaired by 94% (quinine 100 mg/l) or 45% (cinchonine 100 mg/l). The relevance to the clinical situation and the possible mechanisms of such effects are discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cinchona Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Quinine,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Trypan Blue,
http://linkedlifedata.com/resource/pubmed/chemical/cinchonine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-7453
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
949-57
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2164514-Cell-Free System,
pubmed-meshheading:2164514-Chemotaxis,
pubmed-meshheading:2164514-Cinchona Alkaloids,
pubmed-meshheading:2164514-Humans,
pubmed-meshheading:2164514-Luminescent Measurements,
pubmed-meshheading:2164514-Neutrophils,
pubmed-meshheading:2164514-Oxidation-Reduction,
pubmed-meshheading:2164514-Peroxidase,
pubmed-meshheading:2164514-Phagocytosis,
pubmed-meshheading:2164514-Quinine,
pubmed-meshheading:2164514-Superoxides,
pubmed-meshheading:2164514-Trypan Blue
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pubmed:year |
1990
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pubmed:articleTitle |
Effect of quinine and cinchonine on human neutrophils functions in vitro.
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pubmed:affiliation |
INSERM U. 294, CHU Xavier Bichat, Paris, France.
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pubmed:publicationType |
Journal Article,
In Vitro
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