Linked Life Data

2161858

Source:http://linkedlifedata.com/resource/pubmed/id/2161858

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1990-7-16
pubmed:abstractText
We examined a possible role for the plasma membrane potential in signal transduction during cyclic AMP-induced chemotaxis in the cellular slime mold Dictyostelium discoideum. Chemotaxis, cyclic GMP and cyclic AMP responses in cells with a depolarized membrane potential were measured. Cells can be completely depolarized by two different methods: (1) by treatment with azide; this probably causes inhibition of the electrogenic proton pump, which was shown earlier to regulate plasma membrane potential in D. discoideum. (2) By electroporation, which causes the formation of large non-ion-selective pores in the plasma membrane. It was found that in depolarized cells the cyclic AMP-mediated cyclic AMP accumulation was inhibited. In contrast, chemotaxis to a cyclic AMP source was normal; the cyclic AMP-induced accumulation of cyclic GMP, which is known to mediate the chemotactic response, was also not affected. We conclude that membrane-potential-regulated processes, such as voltage-gated ion channels, do not play an essential role in chemotaxis in D. discoideum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
95 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Normal chemotaxis in Dictyostelium discoideum cells with a depolarized plasma membrane potential.
pubmed:affiliation
Cell Biology and Genetics Unit, Leiden University, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't