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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1979-3-13
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pubmed:abstractText |
Isolated simian virus 40 (SV40) and polyoma nucleoprotein complexes contain endonuclease that, under in vitro conditions, converts part (up to 30%) of the covalently closed superhelical DNA to full-length linear rods. The positions of the cleavage sites within the genomes of SV40 and polyoma were determined by digestion with various single-cut restriction endonucleases and subsequent agarose gel electrophoresis of the cleavage products. Both SV40 and polyoma covalently closed superhelical DNA were cleaved open at their respective origins of DNA replication (+/- 75 base pairs). The full-length linear DNA rods whose ends map adjacent to the origin of DNA replication could also be isolated by sodium dodecyl sulfate/phenol extraction both from SV40-infected permissive cells and from purified SV40 virions. These data reveal the presence of a unique structure of the papovavirus chromatin close to the initiation site of DNA replication.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-1122558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-163367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-168578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-17754289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-180531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-180666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-184438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-184963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-185614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-188811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-194707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-198750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-198797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-205546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-206891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-207441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-208060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-208155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4109517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4320050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4331651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4336209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4342055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4343542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4343955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4349757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4365585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/216004-4372308
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5964-8
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:216004-Chromatin,
pubmed-meshheading:216004-Chromosome Mapping,
pubmed-meshheading:216004-DNA, Circular,
pubmed-meshheading:216004-DNA, Viral,
pubmed-meshheading:216004-DNA Replication,
pubmed-meshheading:216004-Endonucleases,
pubmed-meshheading:216004-Kinetics,
pubmed-meshheading:216004-Polyomavirus,
pubmed-meshheading:216004-Simian virus 40,
pubmed-meshheading:216004-Virus Replication
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pubmed:year |
1978
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pubmed:articleTitle |
Origin of DNA replication in papovavirus chromatin is recognized by endogenous endonuclease.
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pubmed:publicationType |
Journal Article
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