Linked Life Data

21596992

Source:http://linkedlifedata.com/resource/pubmed/id/21596992

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6032
pubmed:dateCreated
2011-5-20
pubmed:databankReference
pubmed:abstractText
During protein synthesis, the ribosome controls the movement of tRNA and mRNA by means of large-scale structural rearrangements. We describe structures of the intact bacterial ribosome from Escherichia coli that reveal how the ribosome binds tRNA in two functionally distinct states, determined to a resolution of ~3.2 angstroms by means of x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit site. The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1095-9203
pubmed:author
pubmed:issnType
Electronic
pubmed:day
20
pubmed:volume
332
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
981-4
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:21596992-Anticodon, pubmed-meshheading:21596992-Crystallography, X-Ray, pubmed-meshheading:21596992-Escherichia coli, pubmed-meshheading:21596992-Escherichia coli Proteins, pubmed-meshheading:21596992-Models, Molecular, pubmed-meshheading:21596992-Nucleic Acid Conformation, pubmed-meshheading:21596992-Protein Biosynthesis, pubmed-meshheading:21596992-RNA, Bacterial, pubmed-meshheading:21596992-RNA, Messenger, pubmed-meshheading:21596992-RNA, Ribosomal, 16S, pubmed-meshheading:21596992-RNA, Ribosomal, 23S, pubmed-meshheading:21596992-RNA, Transfer, Amino Acyl, pubmed-meshheading:21596992-RNA, Transfer, Phe, pubmed-meshheading:21596992-Ribosomal Proteins, pubmed-meshheading:21596992-Ribosome Subunits, Large, Bacterial, pubmed-meshheading:21596992-Ribosome Subunits, Small, Bacterial
pubmed:year
2011
pubmed:articleTitle
Structures of the bacterial ribosome in classical and hybrid states of tRNA binding.
pubmed:affiliation
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural