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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1990-6-14
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pubmed:abstractText |
Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-196759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-212483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-2426063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3018293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3023876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3033290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3041045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3054154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3128790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3457477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3469509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3502707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-3785217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-4308055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-6169844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-6281480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-6284984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-6331674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2159569-6678608
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3135-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2159569-Animals,
pubmed-meshheading:2159569-Anti-Bacterial Agents,
pubmed-meshheading:2159569-Antibodies, Monoclonal,
pubmed-meshheading:2159569-Antigens, Polyomavirus Transforming,
pubmed-meshheading:2159569-Cell Cycle,
pubmed-meshheading:2159569-Cell Transformation, Viral,
pubmed-meshheading:2159569-Cells, Cultured,
pubmed-meshheading:2159569-Drug Resistance,
pubmed-meshheading:2159569-Flow Cytometry,
pubmed-meshheading:2159569-Fluorescent Antibody Technique,
pubmed-meshheading:2159569-Genetic Vectors,
pubmed-meshheading:2159569-Gentamicins,
pubmed-meshheading:2159569-Mice,
pubmed-meshheading:2159569-Mice, Inbred Strains,
pubmed-meshheading:2159569-Retroviridae,
pubmed-meshheading:2159569-Simian virus 40
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pubmed:year |
1990
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pubmed:articleTitle |
Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.
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pubmed:affiliation |
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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