Linked Life Data

21567074

Source:http://linkedlifedata.com/resource/pubmed/id/21567074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-6-6
pubmed:abstractText
Injury to articular cartilage is often associated with an inflammatory reaction and frequently results in the development of post-traumatic osteoarthritis (post-traumatic OA). Cell death, inflammation and loss of proteoglycans participate in these mechanisms with p38MAPK being one of the pivotal signaling kinases. Therefore, the interaction of trauma and of the pro-inflammatory cytokine IL-1? was investigated in an in vitro tissue model of human osteoarthritic cartilage. Trauma was induced by impacting cartilage explants with a drop-tower system and its effect was measured in terms of cell survival, gene expression and the release of mediators. In addition, the effect of concomitant IL?1? stimulation and p38MAPK inhibition by SB203580 was investigated. We found a significant decrease in chondrocyte viability after trauma, but no additional effect of IL-1? stimulation. SB203580 had a tendency to improve cell survival suggesting a role for p38 signaling in cell viability after impact in an inflammatory environment. We showed that various mediators are released in response to trauma with or without IL-1? stimulation, differing in composition and time response. Trauma resulted in an increased release of IL-6, whereas TNF-? and IL-1? release was unaffected. Prostaglandin (PG) and NO synthesis pathways were both affected by trauma and/or IL-1?. We demonstrate for the first time an elevated release of prostaglandin D2 (PGD2) by human articular cartilage in response to a single mechanical impact. The up-regulation of mediators was time-dependent, with a more early increase of PGD2 compared to prostaglandin E2 (PGE2) and a late induction of NO by co-stimulation with IL-1? between 6 and 24 h.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1791-244X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-7
pubmed:dateRevised
2011-10-27
pubmed:meshHeading
pubmed-meshheading:21567074-Aged, pubmed-meshheading:21567074-Aged, 80 and over, pubmed-meshheading:21567074-Cartilage, Articular, pubmed-meshheading:21567074-Cell Survival, pubmed-meshheading:21567074-Chondrocytes, pubmed-meshheading:21567074-Cytokines, pubmed-meshheading:21567074-Enzyme Inhibitors, pubmed-meshheading:21567074-Gene Expression Regulation, pubmed-meshheading:21567074-Humans, pubmed-meshheading:21567074-Imidazoles, pubmed-meshheading:21567074-Inflammation Mediators, pubmed-meshheading:21567074-Interleukin-1beta, pubmed-meshheading:21567074-Middle Aged, pubmed-meshheading:21567074-Nitric Oxide Synthase Type II, pubmed-meshheading:21567074-Osteoarthritis, pubmed-meshheading:21567074-Prostaglandin D2, pubmed-meshheading:21567074-Pyridines, pubmed-meshheading:21567074-Signal Transduction, pubmed-meshheading:21567074-Up-Regulation, pubmed-meshheading:21567074-p38 Mitogen-Activated Protein Kinases
pubmed:year
2011
pubmed:articleTitle
Single impact trauma in human early-stage osteoarthritic cartilage: implication of prostaglandin D2 but no additive effect of IL-1? on cell survival.
pubmed:affiliation
Division for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopedics, University of Ulm, Ulm, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't