Source:http://linkedlifedata.com/resource/pubmed/id/21564417
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2011-6-15
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| pubmed:abstractText |
Gene therapy shows promising application in cancer therapy, but the lack of an ideal gene delivery system is still a tough challenge for cancer gene therapy. Previously, we prepared a novel cationic nanogel, heparin-polyethylenimine (HPEI), which had potential application in gene delivery. In the present study, we constructed a plasmid with high expression efficiency of interleukin-15 (IL15) and investigated the effects HPEI-plasmid IL15 (HPEI-pIL15) complexes on the distribution level of the lung. We then evaluated the anticancer effect of HPEI-pIL15 complexes on lung metastases of B16-F10 melanoma and CT26 colon carcinoma. These results demonstrated that intravenous injection of the HPEI-pIL15 complex exhibited the highest plasmid distribution level in the lung compared with that of PEI2K-pIL15 and PEI25K-pIL15, and mice treated with HPEI-pIL15 had a lower tumor metastasis index compared with other treatment groups. Moreover, the number of natural killer cells, which were intermingled among the tumor cells, and the level of tumor necrosis factor-? and interferon-? in the serum also increased in the pIL15-treated mice. Furthermore, the cytotoxic activity of spleen cells also increased significantly in the HPEI-pIL15 group. In addition, induction of apoptosis and inhibition of cell proliferation in lung tumor foci in the HPEI-pIL15 group was observed. Taken together, treating lung metastasis cancer with the HPEI nanogels delivered by plasmid IL15 might be a new and interesting cancer gene therapy protocol.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/NanoGel,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethyleneimine,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
1349-7006
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| pubmed:author |
pubmed-author:ChenZhongwenZ,
pubmed-author:CoxM MMM,
pubmed-author:GouMalingM,
pubmed-author:LiJingJ,
pubmed-author:LiJiongJ,
pubmed-author:LiXiaoleiX,
pubmed-author:LiuXiaoX,
pubmed-author:QianZhiyongZ,
pubmed-author:QiuJiJ,
pubmed-author:ShenGuoboG,
pubmed-author:TengXiuX,
pubmed-author:WangZhenZ,
pubmed-author:WeiYuquanY,
pubmed-author:YangLiL,
pubmed-author:YuChuanjiangC,
pubmed-author:ZhangNannanN,
pubmed-author:ZhangYinbinY,
pubmed-author:ZhouXikunX
|
| pubmed:copyrightInfo |
© 2011 Japanese Cancer Association.
|
| pubmed:issnType |
Electronic
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| pubmed:volume |
102
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1403-9
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| pubmed:meshHeading |
pubmed-meshheading:21564417-Animals,
pubmed-meshheading:21564417-Apoptosis,
pubmed-meshheading:21564417-CHO Cells,
pubmed-meshheading:21564417-Cricetinae,
pubmed-meshheading:21564417-Cricetulus,
pubmed-meshheading:21564417-Female,
pubmed-meshheading:21564417-Gene Therapy,
pubmed-meshheading:21564417-Gene Transfer Techniques,
pubmed-meshheading:21564417-Heparin,
pubmed-meshheading:21564417-Interferon-gamma,
pubmed-meshheading:21564417-Interleukin-15,
pubmed-meshheading:21564417-Lung Neoplasms,
pubmed-meshheading:21564417-Mice,
pubmed-meshheading:21564417-Mice, Inbred BALB C,
pubmed-meshheading:21564417-Mice, Inbred C57BL,
pubmed-meshheading:21564417-Plasmids,
pubmed-meshheading:21564417-Polyethylene Glycols,
pubmed-meshheading:21564417-Polyethyleneimine,
pubmed-meshheading:21564417-Tumor Necrosis Factor-alpha
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| pubmed:year |
2011
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| pubmed:articleTitle |
Antitumoral efficacy by systemic delivery of heparin conjugated polyethylenimine-plasmid interleukin-15 complexes in murine models of lung metastasis.
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| pubmed:affiliation |
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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