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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1979-3-24
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pubmed:abstractText |
After nude-mouse-passage, a carcinoma of the nasopharynx was found to contain a few scattered C-type retrovirus particles. Culture of this nude-mouse-grown material over solid agar allowed the human tumour cells to grow as multicellular spheroids which increased in number by budding and could be subcultured for up to 6 months. Within the spheroids the human tumour cells expressed increased retrovirus replication and large numbers of C-type particles were observed. Treatment with a halogenated pyrimidine further enhanced the virus production. This tissue culture system provided sufficient virus production for the identification of the retrovirus as an endogenous xenotropic murine leukaemia virus and not a human agent.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0020-7136
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-7
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:215558-Animals,
pubmed-meshheading:215558-Bromodeoxyuridine,
pubmed-meshheading:215558-Cell Line,
pubmed-meshheading:215558-Cells, Cultured,
pubmed-meshheading:215558-Culture Media,
pubmed-meshheading:215558-DNA, Viral,
pubmed-meshheading:215558-DNA-Directed RNA Polymerases,
pubmed-meshheading:215558-Female,
pubmed-meshheading:215558-Humans,
pubmed-meshheading:215558-Mice,
pubmed-meshheading:215558-Mice, Nude,
pubmed-meshheading:215558-Middle Aged,
pubmed-meshheading:215558-Nasopharyngeal Neoplasms,
pubmed-meshheading:215558-Retroviridae,
pubmed-meshheading:215558-Time Factors,
pubmed-meshheading:215558-Viral Interference,
pubmed-meshheading:215558-Virus Replication
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pubmed:year |
1979
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pubmed:articleTitle |
Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude-mouse-passaged nasopharyngeal carcinoma.
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pubmed:publicationType |
Journal Article
|