Source:http://linkedlifedata.com/resource/pubmed/id/21539875
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2011-6-6
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| pubmed:abstractText |
A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C(8) reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEMPAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
1873-5169
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
32
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1146-50
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| pubmed:meshHeading |
pubmed-meshheading:21539875-Amino Acid Sequence,
pubmed-meshheading:21539875-Animals,
pubmed-meshheading:21539875-Bodily Secretions,
pubmed-meshheading:21539875-Candida albicans,
pubmed-meshheading:21539875-Chromatography, Reverse-Phase,
pubmed-meshheading:21539875-Cloning, Molecular,
pubmed-meshheading:21539875-Erythrocytes,
pubmed-meshheading:21539875-Escherichia coli,
pubmed-meshheading:21539875-Gene Library,
pubmed-meshheading:21539875-Hemolysis,
pubmed-meshheading:21539875-Humans,
pubmed-meshheading:21539875-Mass Spectrometry,
pubmed-meshheading:21539875-Microbial Sensitivity Tests,
pubmed-meshheading:21539875-Molecular Sequence Data,
pubmed-meshheading:21539875-Oligochaeta,
pubmed-meshheading:21539875-Peptides,
pubmed-meshheading:21539875-Pseudomonas aeruginosa,
pubmed-meshheading:21539875-Rabbits,
pubmed-meshheading:21539875-Skin,
pubmed-meshheading:21539875-Staphylococcus aureus
|
| pubmed:year |
2011
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| pubmed:articleTitle |
A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen).
|
| pubmed:affiliation |
Oncology Department, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China.
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| pubmed:publicationType |
Journal Article
|