Source:http://linkedlifedata.com/resource/pubmed/id/21536845
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-8-2
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| pubmed:abstractText |
The ?(7)-nicotinic ACh receptor (?(7)-nAChR) on sympathetic neurons innervating basilar arteries of pigs crossed bred between Landrace and Yorkshire (LY) is known to mediate nicotine-induced, ?-amyloid (A?)-sensitive nitrergic neurogenic vasodilation. Preliminary studies, however, demonstrated that nicotine-induced cerebral vasodilation in pigs crossbred among Landrace, Yorkshire, and Duroc (LYD) was insensitive to A? and ?-bungarotoxin (?-BGTX). We investigated nAChR subtype on sympathetic neurons innervating LYD basilar arteries. Nicotine-induced relaxation of porcine isolated basilar arteries was examined by tissue bath myography, inward currents on nAChR-expressing oocytes by two-electrode voltage recording, and mRNA and protein expression in the superior cervical ganglion (SCG) and middle cervical ganglion (MCG) by reverse transcription PCR and Western blotting. Nicotine-induced basilar arterial relaxation was not affected by A?, ?-BGTX, and ?-conotoxin IMI (?(7)-nAChR antagonists), or ?-conotoxin AuIB (?(3)?(4)-nAChR antagonist) but was inhibited by tropinone and tropane (?(3)-containing nAChR antagonists) and ?-conotoxin MII (selective ?(3)?(2)-nAChR antagonist). Nicotine-induced inward currents in ?(3)?(2)-nAChR-expressing oocytes were inhibited by ?-conotoxin MII but not by ?-BGTX, A?, or ?-conotoxin AuIB. mRNAs of ?(3)-, ?(7)-, ?(2)-, and ?(4)-subunits were expressed in both SCGs and MCGs with significantly higher mRNAs of ?(3)-, ?(2)-, and ?(4)-subunits than that of ?(7)-subunit. The A?-insensitive sympathetic ?(3)?(2)-nAChR mediates nicotine-induced cerebral nitrergic neurogenic vasodilation in LYD pigs. The different finding from A?-sensitive ?(7)-nAChR in basilar arteries of LY pigs may offer a partial explanation for different sensitivities of individuals to A? in causing diminished cerebral nitrergic vasodilation in diseases involving A?.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid beta-Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoconstrictor Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/nicotinic receptor alpha3beta2
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1522-1539
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
301
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
H344-54
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| pubmed:meshHeading |
pubmed-meshheading:21536845-Amyloid beta-Peptides,
pubmed-meshheading:21536845-Animals,
pubmed-meshheading:21536845-Basilar Artery,
pubmed-meshheading:21536845-Blotting, Western,
pubmed-meshheading:21536845-Dose-Response Relationship, Drug,
pubmed-meshheading:21536845-Electric Stimulation,
pubmed-meshheading:21536845-Female,
pubmed-meshheading:21536845-Humans,
pubmed-meshheading:21536845-Male,
pubmed-meshheading:21536845-Membrane Potentials,
pubmed-meshheading:21536845-Myography,
pubmed-meshheading:21536845-Nicotinic Agonists,
pubmed-meshheading:21536845-Nicotinic Antagonists,
pubmed-meshheading:21536845-Nitrergic Neurons,
pubmed-meshheading:21536845-Oocytes,
pubmed-meshheading:21536845-Patch-Clamp Techniques,
pubmed-meshheading:21536845-RNA, Messenger,
pubmed-meshheading:21536845-Receptors, Nicotinic,
pubmed-meshheading:21536845-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21536845-Superior Cervical Ganglion,
pubmed-meshheading:21536845-Swine,
pubmed-meshheading:21536845-Vasoconstrictor Agents,
pubmed-meshheading:21536845-Vasodilation,
pubmed-meshheading:21536845-Vasodilator Agents,
pubmed-meshheading:21536845-Xenopus
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| pubmed:year |
2011
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| pubmed:articleTitle |
Sympathetic ????-nAChRs mediate cerebral neurogenic nitrergic vasodilation in the swine.
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| pubmed:affiliation |
Institutes of Medical Sciences, College of Medicine, Tzu Chi University, Hualien, Taiwan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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