Source:http://linkedlifedata.com/resource/pubmed/id/21490253
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 9
|
| pubmed:dateCreated |
2011-4-14
|
| pubmed:abstractText |
?-Adrenergic receptors (?ARs) are crucial for maintaining the rate and force of cardiac muscle contraction in vertebrates. Zebrafish (Danio rerio) have one ?1AR gene and two ?2AR genes (?2aAR and ?2bAR). We examined the roles of these receptors in larval zebrafish in vivo by assessing the impact of translational gene knockdown on cardiac function. Zebrafish larvae lacking ?1AR expression by morpholino knockdown displayed lower heart rates than control fish, whereas larvae deficient in both ?2aAR and ?2bAR expression exhibited significantly higher heart rates than controls. These results suggested a potential inhibitory role for one or both ?2AR genes. By using cultured HEK293 cells transfected with zebrafish ?ARs, we demonstrated that stimulation with adrenaline or procaterol (a ?2AR agonist) resulted in an increase in intracellular cAMP levels in cells expressing any of the three zebrafish ?ARs. In comparison with its human ?AR counterpart, zebrafish ?2aAR expressed in HEK293 cells appeared to exhibit a unique binding affinity profile for adrenergic ligands. Specifically, zebrafish ?2aAR had a high binding affinity for phenylephrine, a classical ?-adrenergic receptor agonist. The zebrafish receptors also had distinct ligand binding affinities for adrenergic agonists when compared with human ?ARs in culture, with zebrafish ?2aAR being distinct from human ?2AR and zebrafish ?2bAR. Overall, this study provides insight into the function and evolution of both fish and mammalian ?-adrenergic receptors.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/red fluorescent protein
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1477-9145
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
1
|
| pubmed:volume |
214
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1445-57
|
| pubmed:meshHeading |
pubmed-meshheading:21490253-Aging,
pubmed-meshheading:21490253-Animals,
pubmed-meshheading:21490253-Cell Membrane,
pubmed-meshheading:21490253-GTP-Binding Protein alpha Subunits, Gs,
pubmed-meshheading:21490253-Gene Expression Profiling,
pubmed-meshheading:21490253-Gene Expression Regulation, Developmental,
pubmed-meshheading:21490253-HEK293 Cells,
pubmed-meshheading:21490253-Humans,
pubmed-meshheading:21490253-In Situ Hybridization,
pubmed-meshheading:21490253-Larva,
pubmed-meshheading:21490253-Ligands,
pubmed-meshheading:21490253-Luminescent Proteins,
pubmed-meshheading:21490253-Myocardium,
pubmed-meshheading:21490253-Oligonucleotides, Antisense,
pubmed-meshheading:21490253-RNA, Messenger,
pubmed-meshheading:21490253-Receptors, Adrenergic, beta,
pubmed-meshheading:21490253-Stroke Volume,
pubmed-meshheading:21490253-Zebrafish,
pubmed-meshheading:21490253-Zebrafish Proteins
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
In vivo and in vitro assessment of cardiac beta-adrenergic receptors in larval zebrafish (Danio rerio).
|
| pubmed:affiliation |
Department of Biology, University of Ottawa, Ottawa, ON, Canada, K1N 6N5. sstee057@uottawa.ca
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|