Linked Life Data

2147932

Source:http://linkedlifedata.com/resource/pubmed/id/2147932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-1-28
pubmed:abstractText
Thymocyte cell suspensions, prepared from mice at different ages, were cultured in vitro with human rIL-2. This stimulation resulted in a cell population that contained almost 50% TCR-gamma delta-positive cells if thymocytes were taken from fetal day 17 until just after birth. Analysis of the variable (V gamma) region used by the TCR-gamma delta cells revealed that 90% of them expressed TCR-V gamma 3, and less than 5% expressed TCR-V gamma 2. Cells positive for TCR-alpha beta were barely detectable. If fetal day 18 organ cultured thymus lobes, instead of a cell suspension, were stimulated with IL-2, no rise in the number of TCR-V gamma 3+ or TCR-delta+ cells was observed, whereas a partial outgrowth of TCR-alpha beta+ cells occurred. From day 1 after birth, the number of TCR-gamma delta cells recovered from an IL-2-stimulated thymocyte cell suspension dropped to reach a plateau of 15% of the total cell number, whereas TCR-V gamma 3+ cells became undetectable in older animals. TCR-alpha beta+ cells, on the other hand, quickly rose in cell number after birth. Kinetic analysis showed that the preferential outgrowth of TCR-V gamma 3+ cells in IL-2-stimulated fetal day 18 thymocyte cell suspensions was present from the onset of the culture; a significant proliferation of CD4 or CD8 single positive TCR-alpha beta cells was never observed. This lack of proliferation of TCR-alpha beta cells was not due to inhibition by the activated TCR-V gamma 3+ cells. Throughout the IL-2 culture, one-fourth of the TCR-V gamma 3+ thymocytes was positive for CD8. Analysis of the DNA content and the IL-2 receptor (IL-2R) p55 expression showed that during the first days of culture the TCR-V gamma 3+ cells had a much higher proliferation rate than the TCR-V gamma 3- cells, although TCR-V gamma 3+ IL2R p55+ cells could not be detected. From day 3 to 4 of culture, the proliferation rate of TCR-V gamma 3+ cells equaled that of the rest of the cells and less than 20% of the TCR-V gamma 3+ cells expressed the IL-2R p55. The biologic significance of our findings is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3992-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2147932-Animals, pubmed-meshheading:2147932-Animals, Newborn, pubmed-meshheading:2147932-Antigens, CD4, pubmed-meshheading:2147932-Antigens, CD8, pubmed-meshheading:2147932-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2147932-Cell Differentiation, pubmed-meshheading:2147932-Cell Division, pubmed-meshheading:2147932-Cells, Cultured, pubmed-meshheading:2147932-Interleukin-2, pubmed-meshheading:2147932-Mice, pubmed-meshheading:2147932-Mice, Inbred BALB C, pubmed-meshheading:2147932-Organ Culture Techniques, pubmed-meshheading:2147932-Receptors, Antigen, T-Cell, pubmed-meshheading:2147932-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:2147932-Receptors, Interleukin-2, pubmed-meshheading:2147932-T-Lymphocyte Subsets, pubmed-meshheading:2147932-T-Lymphocytes, pubmed-meshheading:2147932-Thymus Gland
pubmed:year
1990
pubmed:articleTitle
Preferential proliferation of T cell receptor V gamma 3-positive cells in IL-2-stimulated fetal thymocytes.
pubmed:affiliation
Laboratory of Bacteriology, Virology and Immunology, University of Ghent, Belgium.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't