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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
2011-5-16
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pubmed:abstractText |
Quantum dots (QDs) were prepared in genetically engineered Escherichia coli (E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing-thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1873-4863
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
20
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
125-32
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:21458508-Bioreactors,
pubmed-meshheading:21458508-Biotechnology,
pubmed-meshheading:21458508-Cadmium Compounds,
pubmed-meshheading:21458508-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:21458508-Escherichia coli,
pubmed-meshheading:21458508-Genetic Engineering,
pubmed-meshheading:21458508-Microscopy, Electron, Transmission,
pubmed-meshheading:21458508-Peptides,
pubmed-meshheading:21458508-Quantum Dots,
pubmed-meshheading:21458508-Spectrometry, Fluorescence,
pubmed-meshheading:21458508-Sulfides
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pubmed:year |
2011
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pubmed:articleTitle |
Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli.
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pubmed:affiliation |
Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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