Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
|
pubmed:dateCreated |
1990-11-8
|
pubmed:abstractText |
Peripheral blood T lymphocytes from healthy donors were stimulated with Mycobacterium tuberculosis in vitro and afterward analyzed phenotypically. Marked expansion of the gamma/delta T cell population (3- to 21-fold) was observed in 15/21 donors 7 to 10 days after stimulation. In addition to M. tuberculosis, Mycobacterium leprae (six of eight) as well as the gram-positive bacteria, Staphylococcus aureus (two of six), group A streptococci (seven of nine), and Listeria monocytogenes (four of eight) augmented gamma/delta TCR expression in peripheral blood T cells of many donors. gamma/delta T lymphocytes expressed IL-2R and secreted IL-2 upon restimulation with M. tuberculosis. Stimulation with M. tuberculosis evoked specific cytolytic activities in gamma/delta T lymphocytes because: gamma/delta T cells lysed M. tuberculosis pulsed but not unpulsed targets; high concentrations of TCR delta 1 mAb facilitated killing of unpulsed target cells; and low doses of anti-TCR delta 1 mAb blocked killing of pulsed targets. Furthermore, gamma/delta T cells from four donors, after activation with M. tuberculosis or with group A streptococci, respectively, only lysed targets pulsed with the homologous agents, whereas in other donors some cross-reactivity was observed. We conclude that, upon contact with mycobacteria and perhaps other microorganisms, gamma/delta T cells are activated which contribute to immunity against infection via IL-2 secretion and specific target cell lysis.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
145
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2434-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2145360-Antigens, Bacterial,
pubmed-meshheading:2145360-Cells, Cultured,
pubmed-meshheading:2145360-Humans,
pubmed-meshheading:2145360-Interleukin-2,
pubmed-meshheading:2145360-Listeria monocytogenes,
pubmed-meshheading:2145360-Lymphocyte Activation,
pubmed-meshheading:2145360-Mycobacterium leprae,
pubmed-meshheading:2145360-Mycobacterium tuberculosis,
pubmed-meshheading:2145360-Receptors, Antigen, T-Cell,
pubmed-meshheading:2145360-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:2145360-Receptors, Interleukin-2,
pubmed-meshheading:2145360-Streptococcus pyogenes,
pubmed-meshheading:2145360-T-Lymphocyte Subsets,
pubmed-meshheading:2145360-T-Lymphocytes, Cytotoxic
|
pubmed:year |
1990
|
pubmed:articleTitle |
Target cell lysis and IL-2 secretion by gamma/delta T lymphocytes after activation with bacteria.
|
pubmed:affiliation |
Department of Medical Microbiology and Immunology, University of Ulm, FRG.
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|