|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2011-5-16
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Vibrio cholerae relies on two main virulence factors--toxin-coregulated pilus (TCP) and cholera toxin--to cause the gastrointestinal disease cholera. TCP is a type IV pilus that mediates bacterial autoagglutination and colonization of the intestine. TCP is encoded by the tcp operon, which also encodes TcpF, a protein of unknown function that is secreted by V. cholerae in a TCP-dependent manner. Although TcpF is not required for TCP biogenesis, a tcpF mutant has a colonization defect in the infant mouse cholera model that is as severe as a pilus mutant. Furthermore, TcpF antisera protect against V. cholerae infection. TcpF has no apparent sequence homology to any known protein. Here, we report the de novo X-ray crystal structure of TcpF and the identification of an epitope that is critical for its function as a colonization factor. A monoclonal antibody recognizing this epitope is protective against V. cholerae challenge and adds to the protection provided by an anti-TcpA antibody. These data suggest that TcpF has a novel function in V. cholerae colonization and define a region crucial for this function.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1089-8638
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
3
|
|
pubmed:volume |
409
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
146-58
|
|
pubmed:meshHeading |
pubmed-meshheading:21440558-Animals,
pubmed-meshheading:21440558-Antibodies, Monoclonal,
pubmed-meshheading:21440558-Antigens, Bacterial,
pubmed-meshheading:21440558-Bacterial Proteins,
pubmed-meshheading:21440558-Cholera,
pubmed-meshheading:21440558-Crystallography, X-Ray,
pubmed-meshheading:21440558-Disease Models, Animal,
pubmed-meshheading:21440558-Female,
pubmed-meshheading:21440558-Immunoblotting,
pubmed-meshheading:21440558-Intestines,
pubmed-meshheading:21440558-Mice,
pubmed-meshheading:21440558-Mice, Inbred BALB C,
pubmed-meshheading:21440558-Mutagenesis, Site-Directed,
pubmed-meshheading:21440558-Mutation,
pubmed-meshheading:21440558-Peptide Fragments,
pubmed-meshheading:21440558-Peptide Library,
pubmed-meshheading:21440558-Protein Conformation,
pubmed-meshheading:21440558-Survival Rate,
pubmed-meshheading:21440558-Transcription Factors,
pubmed-meshheading:21440558-Vibrio cholerae
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Crystal structure of the Vibrio cholerae colonization factor TcpF and identification of a functional immunogenic site.
|
|
pubmed:affiliation |
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
