Source:http://linkedlifedata.com/resource/pubmed/id/21435045
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2011-6-10
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693760,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693761,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693762,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693763,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693764,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693765,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693766,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/HQ693767
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pubmed:abstractText |
In flowering plants, class-B floral homeotic genes encode MADS-domain transcription factors, which are key in the specification of petal and stamen identity, and have two ancient clades: DEF-like and GLO-like genes. Many species have one gene of each clade, but orchids have typically four DEF-like genes, representing ancient gene clades 1, 2, 3 and 4. We tested the 'orchid code', a combinatorial genetic model suggesting that differences between the organs of the orchid perianth (outer tepals, inner lateral tepals and labellum) are generated by the combinatorial differential expression of four DEF-like genes. Our experimental test involves highly sensitive and specific measurements, with qRT-PCR of the expression of DEF- and GLO-like genes from the distantly related Vanilla planifolia and Phragmipedium longifolium, as well as from wild-type and peloric Phalaenopsis hybrid flowers. Our findings support the first 'orchid code' hypothesis, in that absence of clade-3 and -4 gene expression distinguishes the outer tepals from the inner tepals. In contrast to the original hypothesis, however, mRNA of both clade-3 and -4 genes accumulates in wild-type inner lateral tepals and the labellum, and in labellum-like inner lateral tepals of peloric flowers, albeit in different quantities. Our data suggest a revised hypothesis where high levels of clade-1 and -2, and low levels of clade-3 and -4, gene expression specify inner lateral tepals, whereas labellum development requires low levels of clade-1 and -2 expression and high levels of clade-3 and -4 expression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1365-313X
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pubmed:author | |
pubmed:copyrightInfo |
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.
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pubmed:issnType |
Electronic
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1008-19
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pubmed:meshHeading |
pubmed-meshheading:21435045-Amino Acid Sequence,
pubmed-meshheading:21435045-Evolution, Molecular,
pubmed-meshheading:21435045-Flowers,
pubmed-meshheading:21435045-Gene Duplication,
pubmed-meshheading:21435045-Gene Expression Regulation, Developmental,
pubmed-meshheading:21435045-Gene Expression Regulation, Plant,
pubmed-meshheading:21435045-Genes, Homeobox,
pubmed-meshheading:21435045-Genes, Plant,
pubmed-meshheading:21435045-MADS Domain Proteins,
pubmed-meshheading:21435045-Molecular Sequence Data,
pubmed-meshheading:21435045-Orchidaceae,
pubmed-meshheading:21435045-Phylogeny,
pubmed-meshheading:21435045-Plant Proteins,
pubmed-meshheading:21435045-RNA, Plant,
pubmed-meshheading:21435045-Sensitivity and Specificity,
pubmed-meshheading:21435045-Transcription Factors
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pubmed:year |
2011
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pubmed:articleTitle |
Conserved differential expression of paralogous DEFICIENS- and GLOBOSA-like MADS-box genes in the flowers of Orchidaceae: refining the 'orchid code'.
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pubmed:affiliation |
Department of Genetics, Friedrich Schiller University Jena, Philosophenweg 12, 07743 Jena, Germany. mariana.mondragon@biologie.uni-regensburg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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