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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-9-7
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pubmed:abstractText |
Requirements for the induction of human cytolytic T-lymphocyte (CTL) activity were studied in a monocyte-free T-cell activation system that uses immobilized anti-CD3 monoclonal antibodies (mAb) as a stimulus. Alloreactive CTL with specificity for HLA-A and -B locus antigens could be demonstrated within 2 days after the initiation of activation. CTL induction in purified T cells initiated by an optimal concentration of immobilized anti-CD3 mAb was not enhanced by the addition of monocytes or exogeneous cytokines, whereas addition of anti-CD25 mAb largely blocked the response. Upon suboptimal anti-CD3 mAb stimulation, addition of recombinant interleukin (rIL)-2, rIL-1 and rIL-4, but not recombinant interferon-gamma (IFN-gamma) or rIL-6, potentiated the development of CTL activity. Finally it was shown that immobilized anti-CD3 mAb induced significant levels of CTL activity in both purified CD4+ and CD8+ cells. This study indicates that the requirement for cytokines in the differentiation of CTL precursors depends on the strength of the activation signal delivered through the T-cell receptor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2456372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2463100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2502419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2525425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2580895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2649138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2784461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2838549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2935404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2960543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2968520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-2972366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3082972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3086885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3087758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3097123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3102594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3108668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3110277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3117878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3141268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3158532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3257241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3259965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3260543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3261753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3261754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3278321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3320204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3489775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3493155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3494950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3496227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3500261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3500262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3871453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-3872800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2143171-6161959
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
357-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2143171-Antibodies, Monoclonal,
pubmed-meshheading:2143171-Antigens, CD,
pubmed-meshheading:2143171-Antigens, CD3,
pubmed-meshheading:2143171-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2143171-Biological Factors,
pubmed-meshheading:2143171-Cells, Cultured,
pubmed-meshheading:2143171-Cytokines,
pubmed-meshheading:2143171-Humans,
pubmed-meshheading:2143171-Lymphocyte Activation,
pubmed-meshheading:2143171-Membrane Glycoproteins,
pubmed-meshheading:2143171-Receptors, Antigen, T-Cell,
pubmed-meshheading:2143171-Recombinant Proteins,
pubmed-meshheading:2143171-T-Lymphocytes,
pubmed-meshheading:2143171-T-Lymphocytes, Cytotoxic
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pubmed:year |
1990
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pubmed:articleTitle |
Generation of alloreactive cytolytic T lymphocytes by immobilized anti-CD3 monoclonal antibodies. Analysis of requirements for human cytolytic T-lymphocyte differentiation.
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pubmed:affiliation |
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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