|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
13
|
|
pubmed:dateCreated |
2011-7-25
|
|
pubmed:abstractText |
Human RNA polymerase (Pol) III-transcribed genes are thought to share a simple termination signal constituted by four or more consecutive thymidine residues in the coding DNA strand, just downstream of the RNA 3'-end sequence. We found that a large set of human tRNA genes (tDNAs) do not display any T(?4) stretch within 50?bp of 3'-flanking region. In vitro analysis of tDNAs with a distanced T(?4) revealed the existence of non-canonical terminators resembling degenerate T(?5) elements, which ensure significant termination but at the same time allow for the production of Pol III read-through pre-tRNAs with unusually long 3' trailers. A panel of such non-canonical signals was found to direct transcription termination of unusual Pol III-synthesized viral pre-miRNA transcripts in gammaherpesvirus 68-infected cells. Genome-wide location analysis revealed that human Pol III tends to trespass into the 3'-flanking regions of tDNAs, as expected from extensive terminator read-through. The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3'-trailer sequences with the potential to contribute novel functional ncRNAs.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-10024448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-10843998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-10964558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-11118217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-12554662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-12711671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-12713904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-1314952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-15347814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-15632064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-15667936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-15782219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-15788403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-16362040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-16787917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-17274687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-17977614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-18573085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-18790808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-18974046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-19487567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-19931532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-19933153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-19940126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-19948768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20129062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20154270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20181738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20413673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20498841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-20890107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-2411938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-2443712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-2585499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-2828928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-3902472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-6194892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-6203900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-6263489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-6693417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-7498793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-7545436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-7877593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-8165140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-8308883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-8565070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-9023104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-9159473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-9223479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-954087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21421562-9660958
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1362-4962
|
|
pubmed:author |
pubmed-author:DiebelKevin WKW,
pubmed-author:DieciGiorgioG,
pubmed-author:HernandezNouriaN,
pubmed-author:OrioliAndreaA,
pubmed-author:PascaliChiaraC,
pubmed-author:PercudaniRiccardoR,
pubmed-author:PrazVivianeV,
pubmed-author:QuartararoJadeJ,
pubmed-author:RomascanoDavidD,
pubmed-author:TeichmannMartinM,
pubmed-author:van DykLinda FLF
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
39
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5499-512
|
|
pubmed:meshHeading |
pubmed-meshheading:21421562-3' Flanking Region,
pubmed-meshheading:21421562-Animals,
pubmed-meshheading:21421562-Cell Line,
pubmed-meshheading:21421562-HeLa Cells,
pubmed-meshheading:21421562-Humans,
pubmed-meshheading:21421562-Mice,
pubmed-meshheading:21421562-RNA, Transfer,
pubmed-meshheading:21421562-RNA Polymerase III,
pubmed-meshheading:21421562-Sequence Analysis, DNA,
pubmed-meshheading:21421562-Terminator Regions, Genetic,
pubmed-meshheading:21421562-Transcription, Genetic
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.
|
|
pubmed:affiliation |
Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Viale G.P. Usberti 23/A, 43100 Parma, Italy.
|
|
pubmed:publicationType |
Journal Article
|
