Linked Life Data

21400673

Source:http://linkedlifedata.com/resource/pubmed/id/21400673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-3-14
pubmed:abstractText
Whole-genome sequencing, also known as deep sequencing, is becoming a more affordable and efficient way to identify SNP mutations, deletions, and insertions in DNA sequences across several different strains. Two major obstacles preventing the widespread use of deep sequencers are the costs involved in services used to prepare DNA libraries for sequencing and the overall accuracy of the sequencing data. This unit describes the preparation of DNA libraries for multiplexed paired-end sequencing using the Illumina GA series sequencer. Self-preparation of DNA libraries can help reduce overall expenses, especially if optimization is required for the different samples, and use of the Illumina GA Sequencer can improve the quality of the data.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1934-8533
pubmed:author
pubmed:copyrightInfo
© 2011 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 1E.4
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Preparing DNA libraries for multiplexed paired-end deep sequencing for Illumina GA sequencers.
pubmed:affiliation
Dartmouth Medical School, Hanover, New Hampshire, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural