Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-4-19
pubmed:abstractText
The aim of this study was to test the hypothesis that nuclear factor-kappa B (NF-?B) is involved in TRPV4-NO pathway in thermal hyperalgesia following chronic compression of the dorsal root ganglion (DRG) (the procedure hereafter termed CCD) in rat. Intrathecal administration of two NF-?B inhibitors, pyrrolidine dithiocarbamate (PDTC; 10(-1) to 10(-2)M) and BAY (100-50 ?M), both induced significantly dose-dependent increase in the paw withdrawal latency (PWL) and decrease in nitric oxide (NO) content in DRG when compared with control rats. Pretreatment with 4?-phorbol 12,13-didecanoate (4?-PDD, transient receptor potential vanilloid 4 (TRPV4) synthetic activator, 1 nm) attenuated the suppressive effects of PDTC (10(-1)M) and BAY (100 ?M) on CCD-induced thermal hyperalgesia and NO production. In addition, Western blot analysis indicated that CCD rats exhibited nuclear NF-?B protein expression and low levels of cytoplasmic inhibitory-kappa B (I-?B) expression; the increase in NF-?B expression and decrease in I-?B expression were reversed after intrathecal injection of PDTC. In conclusion, our data suggested that NF-?B could be involved in TRPV4-NO pathway in CCD-induced thermal hyperalgesia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-methylphenylsulfonyl)-2-propene..., http://linkedlifedata.com/resource/pubmed/chemical/4alpha-phorbol 12,13-didecanone, http://linkedlifedata.com/resource/pubmed/chemical/Analgesics, http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitriles, http://linkedlifedata.com/resource/pubmed/chemical/Phorbols, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines, http://linkedlifedata.com/resource/pubmed/chemical/Sulfones, http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels, http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates, http://linkedlifedata.com/resource/pubmed/chemical/Trpv4 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1872-7549
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
221
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-24
pubmed:meshHeading
pubmed-meshheading:21356247-Analgesics, pubmed-meshheading:21356247-Animals, pubmed-meshheading:21356247-Disease Models, Animal, pubmed-meshheading:21356247-Dose-Response Relationship, Drug, pubmed-meshheading:21356247-Ganglia, Spinal, pubmed-meshheading:21356247-Hyperalgesia, pubmed-meshheading:21356247-I-kappa B Proteins, pubmed-meshheading:21356247-Male, pubmed-meshheading:21356247-NF-kappa B, pubmed-meshheading:21356247-Nerve Compression Syndromes, pubmed-meshheading:21356247-Neuralgia, pubmed-meshheading:21356247-Nitric Oxide, pubmed-meshheading:21356247-Nitriles, pubmed-meshheading:21356247-Phorbols, pubmed-meshheading:21356247-Pyrrolidines, pubmed-meshheading:21356247-Rats, pubmed-meshheading:21356247-Rats, Wistar, pubmed-meshheading:21356247-Signal Transduction, pubmed-meshheading:21356247-Sulfones, pubmed-meshheading:21356247-TRPV Cation Channels, pubmed-meshheading:21356247-Thiocarbamates
pubmed:year
2011
pubmed:articleTitle
Nuclear factor-kappa B mediates TRPV4-NO pathway involved in thermal hyperalgesia following chronic compression of the dorsal root ganglion in rats.
pubmed:affiliation
Department of Physical Medicine & Rehabilitation, Qilu Hospital, Medical School of Shandong University, Jinan 250012, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't