|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
2011-7-7
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Lens epithelium-derived growth factor (LEDGF) or p75 is a co-activator of general transcription and also involved in insertion of human immunodeficiency virus type I (HIV-1) cDNA into host cell genome, which occurs preferentially to active transcription units. These phenomena may share an underlying molecular mechanism in common. We report here that LEDGF/p75 binds negatively supercoiled DNA selectively over unconstrained DNA. We identified a novel DNA-binding domain in the protein and termed it 'supercoiled DNA-recognition domain' (SRD). Recombinant protein fragments containing SRD showed a preferential binding to supercoiled DNA in vitro. SRD harbors a characteristic cluster of lysine and glutamic/aspartic acid residues. A polypeptide mimicking the cluster (K(9)E(9)K(9)) also showed this specificity, suggesting that the cluster is an essential element for the supercoil recognition. eGFP-tagged LEDGF/p75 expressed in the nucleus distributed partially in transcriptionally active regions that were identified by immunostaining of methylated histone H3 (H3K4me3) or incorporation of Br-UTP. This pattern of localization was observed with SRD alone but abolished if the protein lacked SRD. Thus, these results imply that LEDGF/p75 guides its binding partners, including HIV-1 integrase, to the active transcription site through recognition of negative supercoils generated around it.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-10398197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-10623627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-10856157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11106659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11170002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11294645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11395412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11433033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-11512661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-12202041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-12407101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-12796494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-12805549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15022305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15107489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15308744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15314653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15371438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15563464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15797927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-15800630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-16311605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-16403635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-16735438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-16793062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-16959972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-17070642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-17391707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-17639082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-17676063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-18092005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-18193062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-18598942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-18799576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-19244240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-19250904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-19864417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-19865164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-19889764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-20400950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-20484370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-20547484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-20554522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-3019334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-3366776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-4092697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-7919794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-8320255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-8509422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21345933-9822615
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1362-4962
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
39
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5067-81
|
|
pubmed:meshHeading |
pubmed-meshheading:21345933-Animals,
pubmed-meshheading:21345933-Base Sequence,
pubmed-meshheading:21345933-Cell Nucleus,
pubmed-meshheading:21345933-DNA, Superhelical,
pubmed-meshheading:21345933-HeLa Cells,
pubmed-meshheading:21345933-Humans,
pubmed-meshheading:21345933-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:21345933-Molecular Sequence Data,
pubmed-meshheading:21345933-Nuclear Proteins,
pubmed-meshheading:21345933-Protein Structure, Tertiary,
pubmed-meshheading:21345933-Rats,
pubmed-meshheading:21345933-Rats, Sprague-Dawley,
pubmed-meshheading:21345933-Transcription, Genetic
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Nuclear protein LEDGF/p75 recognizes supercoiled DNA by a novel DNA-binding domain.
|
|
pubmed:affiliation |
Department of Neurogenomics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.
|
