Linked Life Data

21345352

Source:http://linkedlifedata.com/resource/pubmed/id/21345352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-4-18
pubmed:abstractText
Noroviruses are implicated in many worldwide institutional, food and waterborne outbreaks each year. Genetic typing of isolates is valuable for monitoring outbreak spread as well as variation in circulating strains. Microarrays have the potential to provide rapid genotype information for norovirus samples. The NoroChip v3.0 provides an oligonucleotide hybridization platform to screen for over 600 potential interactions in each experiment. The NoroChip v3.0 was developed at Health Canada and validated in seven international partner laboratories. Each laboratory validated the NoroChip v3.0 using norovirus amplicons routinely characterized in their testing protocols. Fragments from the capsid region (region C) and a 2.4 kb amplicon spanning polymerase and capsid sequences (region AD) were validated in six of the partner laboratories and provided correct genogroup typing information (GI or GII) when hybridized to the NoroChip v3.0. Results indicate that the current limiting factor for implementing the NoroChip v3.0 as a strain typing tool is the difficulty obtaining a long, specific amplicon from all circulating norovirus strains. Data obtained with the longer region AD amplicon provided the best discrimination between norovirus strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1879-0984
pubmed:author
pubmed:copyrightInfo
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-50
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products.
pubmed:affiliation
Bureau of Microbial Hazards, Health Canada, Ottawa, Canada. Kirsten.Mattison@hc-sc.gc.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Multicenter Study, Validation Studies