Source:http://linkedlifedata.com/resource/pubmed/id/21339366
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2011-3-22
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| pubmed:abstractText |
Binding of immunoglobulin protein (BiP) is a major molecular chaperone localized in endoplasmic reticulum (ER). It has been demonstrated to interact with nascent Ig. However, contrary to other ER-resident heat shock proteins such as gp96, calreticulin, and ORP150, it is not clear whether tumor-derived BiP plays a role in inducing antitumor immunity. In this study, we show that the tumor-derived secreted form of BiP is capable of inducing antitumor CD8(+) T cell responses. We constructed an ER-retention signal KDEL-deleted mutant of BiP cDNA and transfected it to tumor cells, which resulted in continuous secretion of tumor-derived BiP into the extracellular milieu. We show that this secreted BiP is taken up by bone marrow-derived dendritic cells, and thereafter BiP-associated Ag peptide is cross-presented in association with MHC class I molecules, resulting in elicitation of an Ag-specific CD8(+) T cell response and antitumor effect. This strategy to boost antitumor immune responses shows that a tumor could be its own cellular vaccine via gene modification of the secretion of the tumor Ag-BiP complex.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1550-6606
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
1
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| pubmed:volume |
186
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4325-30
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:21339366-Amino Acid Sequence,
pubmed-meshheading:21339366-Animals,
pubmed-meshheading:21339366-Antigens, Neoplasm,
pubmed-meshheading:21339366-Binding Sites, Antibody,
pubmed-meshheading:21339366-Cell Line, Tumor,
pubmed-meshheading:21339366-Cells, Cultured,
pubmed-meshheading:21339366-Colonic Neoplasms,
pubmed-meshheading:21339366-Cross-Priming,
pubmed-meshheading:21339366-Epitopes,
pubmed-meshheading:21339366-Female,
pubmed-meshheading:21339366-Heat-Shock Proteins,
pubmed-meshheading:21339366-Lung Neoplasms,
pubmed-meshheading:21339366-Mice,
pubmed-meshheading:21339366-Mice, Inbred BALB C,
pubmed-meshheading:21339366-Mice, Inbred C3H,
pubmed-meshheading:21339366-Mice, Inbred C57BL,
pubmed-meshheading:21339366-Mice, Nude,
pubmed-meshheading:21339366-Molecular Sequence Data,
pubmed-meshheading:21339366-Thymoma
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Tumor-produced secreted form of binding of immunoglobulin protein elicits antigen-specific tumor immunity.
|
| pubmed:affiliation |
Department of Pathology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8556, Japan. ytamura@sapmed.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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