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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-2-14
pubmed:abstractText
The use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4 × 10(6)??propagules ml(-1)) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill any significant number. Infested tomato roots that were inoculated with mycelia plus pectinase (20?U?ml(-1)) had over 50% tubercles dead one week after treatment. Those inoculated with mycelia plus cellulase (20?U?ml(-1)) had above 60% mortality. Mixtures of mycelial plus the two enzymes (10?U?ml(-1) of each enzyme) showed synergy. The activity catalyzed by an enzyme is a measure of the amount of enzyme present. It was shown that, in a 1?mg (10?U?mg(-1)) cellulase used, 0.055?mg pectinase (1.1?U?mg(-1)) is present. This explains why mycelial plus cellulase mix contends with mycelial plus the two enzymes.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1687-9198
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2010
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273264
pubmed:dateRevised
2011-7-25
pubmed:year
2010
pubmed:articleTitle
Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape.
pubmed:affiliation
Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa.
pubmed:publicationType
Journal Article