Linked Life Data

21307596

Source:http://linkedlifedata.com/resource/pubmed/id/21307596

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-2-25
pubmed:abstractText
The effects of ?-5 gliadin on wheat-dependent exercise-induced anaphylaxis (WDEIA) were investigated by using a mouse model. The gliadin fraction was prepared as a 70% ethanol-soluble solution, and ?-5 gliadin was purified by chromatography. Purified ?-5 gliadin was run on SDS-PAGE gel to reveal three bands with a molecular mass of 53-60 kDa and had the characteristic N-terminal sequence of ?-5 gliadin. The mice were sensitized to the gliadin fraction, and the anaphylactic response was assessed by measuring the body temperature and voluntary physical activity. An oral administration of ?-5 gliadin evoked a significant drop in both the body temperature and voluntary physical activity, similar to the effects of the whole gliadin fraction. ELISA and immunoblotting analyses revealed that the IgE expression from sensitized mice reacted most strongly to ?-5 gliadin. Taken together, these results indicate ?-5 gliadin to be a major allergen responsible for stimulating WDEIA in mice, with the characteristic potential for stimulating IgE production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1347-6947
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Impact of ?-5 gliadin on wheat-dependent exercise-induced anaphylaxis in mice.
pubmed:affiliation
Department of Clinical Nutrition, Kawasaki University of Medical Welfare, Kurashiki, Okayama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't