pubmed-article:2129257 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0001483 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0319205 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0062637 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C0205549 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:2129257 | lifeskim:mentions | umls-concept:C1627932 | lld:lifeskim |
pubmed-article:2129257 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:2129257 | pubmed:dateCreated | 1991-8-26 | lld:pubmed |
pubmed-article:2129257 | pubmed:abstractText | After immunization of mice with purified hexon (the main capsid antigen) of bovine adenovirus serotype BAV3 we have obtained a set of 16 individual hybridoma clones producing MAb's against BAV3 hexon. All MAb's were shown to belong to immunoglobulin G class. Specificity of the most avid MAb marked B3Hx-1 was tested on a panel of representative hexon antigens from 16 adenovirus serotypes of human and animal origin using several immunoassays. In Western blot analysis the MAb B3Hx-1 reacted only with native (trimeric) form of hexon protein and not with denaturated hexon polypeptide chains. The epitope defined by B3Hx-1 appeared stable against SDS at ambient temperature and against chloramine-promoted iodination. The specificity of the epitope was characterized as almost genus-crossreactive: it was absent from hexons of avian and of bovine subgroup 2 adenovirus serotypes and present in most hexons of bovine, canine, simian and human adenoviruses tested. Within the latter group its expression was weak or absent only for human subgenus C serotypes. Several variants of sandwich-type ELISA were developed using MAb B3Hx-1 and different polyclonal antibodies against hexons of mammalian adenoviruses. The level of hexon detection for different adenovirus serotypes varied in range 10(-9) to 10(-8) g per ml. | lld:pubmed |
pubmed-article:2129257 | pubmed:language | eng | lld:pubmed |
pubmed-article:2129257 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2129257 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2129257 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2129257 | pubmed:issn | 0231-4622 | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:TikchonenkoT... | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:KiselevaE KEK | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:KhilkoS NSN | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:IsakovaV RVR | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:KirasovaM AMA | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:Zelivyanskaya... | lld:pubmed |
pubmed-article:2129257 | pubmed:author | pubmed-author:GrigoryevV... | lld:pubmed |
pubmed-article:2129257 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2129257 | pubmed:volume | 37 | lld:pubmed |
pubmed-article:2129257 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2129257 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2129257 | pubmed:pagination | 307-14 | lld:pubmed |
pubmed-article:2129257 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
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pubmed-article:2129257 | pubmed:year | 1990 | lld:pubmed |
pubmed-article:2129257 | pubmed:articleTitle | Characterization of a series of monoclonal antibodies specific for bovine adenovirus serotype BAV3 hexon antigen and their use in an ELISA for detection of adenoviral hexons. | lld:pubmed |
pubmed-article:2129257 | pubmed:affiliation | Research Institute for Agricultural Biotechnology, Moscow, USSR. | lld:pubmed |
pubmed-article:2129257 | pubmed:publicationType | Journal Article | lld:pubmed |