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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1991-8-26
|
| pubmed:abstractText |
After immunization of mice with purified hexon (the main capsid antigen) of bovine adenovirus serotype BAV3 we have obtained a set of 16 individual hybridoma clones producing MAb's against BAV3 hexon. All MAb's were shown to belong to immunoglobulin G class. Specificity of the most avid MAb marked B3Hx-1 was tested on a panel of representative hexon antigens from 16 adenovirus serotypes of human and animal origin using several immunoassays. In Western blot analysis the MAb B3Hx-1 reacted only with native (trimeric) form of hexon protein and not with denaturated hexon polypeptide chains. The epitope defined by B3Hx-1 appeared stable against SDS at ambient temperature and against chloramine-promoted iodination. The specificity of the epitope was characterized as almost genus-crossreactive: it was absent from hexons of avian and of bovine subgroup 2 adenovirus serotypes and present in most hexons of bovine, canine, simian and human adenoviruses tested. Within the latter group its expression was weak or absent only for human subgenus C serotypes. Several variants of sandwich-type ELISA were developed using MAb B3Hx-1 and different polyclonal antibodies against hexons of mammalian adenoviruses. The level of hexon detection for different adenovirus serotypes varied in range 10(-9) to 10(-8) g per ml.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/hexon capsid protein, Adenovirus
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0231-4622
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
307-14
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2129257-Adenoviridae,
pubmed-meshheading:2129257-Animals,
pubmed-meshheading:2129257-Antibodies, Monoclonal,
pubmed-meshheading:2129257-Antibodies, Viral,
pubmed-meshheading:2129257-Antibody Affinity,
pubmed-meshheading:2129257-Antibody Specificity,
pubmed-meshheading:2129257-Antigens, Viral,
pubmed-meshheading:2129257-Birds,
pubmed-meshheading:2129257-Capsid,
pubmed-meshheading:2129257-Capsid Proteins,
pubmed-meshheading:2129257-Cattle,
pubmed-meshheading:2129257-Dogs,
pubmed-meshheading:2129257-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2129257-Haplorhini,
pubmed-meshheading:2129257-Humans,
pubmed-meshheading:2129257-Hybridomas,
pubmed-meshheading:2129257-Mice,
pubmed-meshheading:2129257-Mice, Inbred BALB C,
pubmed-meshheading:2129257-Species Specificity
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Characterization of a series of monoclonal antibodies specific for bovine adenovirus serotype BAV3 hexon antigen and their use in an ELISA for detection of adenoviral hexons.
|
| pubmed:affiliation |
Research Institute for Agricultural Biotechnology, Moscow, USSR.
|
| pubmed:publicationType |
Journal Article
|