Linked Life Data

21291881

Source:http://linkedlifedata.com/resource/pubmed/id/21291881

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2011-3-4
pubmed:abstractText
We have demonstrated that the activation of 5-hydroxytryptamine (5-HT) receptor 3 in the submucosal plexus suppresses 5-HT-induced colonic ion secretion by increasing submucosal somatostatin release. A number of psychological and physical stresses have impacts on the intestinal mucosal functions, including secretion and the epithelial barrier. Whether the 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway in the rat distal colon is involved in the stress process is still unknown. The present study aims to investigate the effect of the water-immersion restraint stress on this inhibitory pathway and its underlying mechanisms. Mucosa/submucosa preparations from the rat distal colon were mounted in the Ussing chambers for the measurement of short-circuit current (I(SC)). Real-time PCR and western blot were performed to study the expression of the 5-HT(3) receptor, 5-HT(4) receptor, and somatostatin receptor 2. Radioimmunoassay was used to measure somatostatin release. After 2h of water-immersion restraint stress, the membrane resistance (Rte) of rat mucosa/submucosa preparations was significantly decreased, but the baseline I(SC) and 5-HT-induced I(SC) responses were significantly increased. The protein expression of the submucosal 5-HT(3) receptors and mucosal somatostatin receptor 2 were down-regulated, and the 5-HT-induced somatostatin release from the mucosa/submucosa preparations was significantly reduced in the stress group. Taken together, these results suggest that the 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway is suppressed in the water-immersion restraint stressed rats, which may contribute to the acute stress-induced increase in colonic secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1879-0712
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
656
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
94-100
pubmed:meshHeading
pubmed-meshheading:21291881-Animals, pubmed-meshheading:21291881-Colon, pubmed-meshheading:21291881-Electric Conductivity, pubmed-meshheading:21291881-Electrophysiological Processes, pubmed-meshheading:21291881-Gene Expression Regulation, pubmed-meshheading:21291881-Immersion, pubmed-meshheading:21291881-Intestinal Mucosa, pubmed-meshheading:21291881-Male, pubmed-meshheading:21291881-Rats, pubmed-meshheading:21291881-Rats, Sprague-Dawley, pubmed-meshheading:21291881-Receptors, Serotonin, 5-HT3, pubmed-meshheading:21291881-Receptors, Serotonin, 5-HT4, pubmed-meshheading:21291881-Receptors, Somatostatin, pubmed-meshheading:21291881-Restraint, Physical, pubmed-meshheading:21291881-Serotonin, pubmed-meshheading:21291881-Serotonin 5-HT3 Receptor Antagonists, pubmed-meshheading:21291881-Somatostatin, pubmed-meshheading:21291881-Stress, Psychological, pubmed-meshheading:21291881-Tropanes, pubmed-meshheading:21291881-Water
pubmed:year
2011
pubmed:articleTitle
Colonic submucosal 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway is suppressed in water-immersion restraint stressed rats.
pubmed:affiliation
Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing (100069), PR China.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't