Linked Life Data

21289055

Source:http://linkedlifedata.com/resource/pubmed/id/21289055

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-4-5
pubmed:abstractText
The transcription factor NF-?B has been found critical to the pathogenesis of renal ischemia-reperfusion injury, which is a major cause of acute kidney injury (AKI). Activation of NF-?B is dependent upon the activation of the specific inhibitory ?B kinase (IKK) subunit IKK?. Here, we investigate whether small interfering RNA (siRNA) targeting IKK? protects rats from renal ischemia- reperfusion injury in vivo. Renal ischemia-reperfusion injury was induced by clamping the renal artery for 45 min. Rats were treated before ischemia with IKK? siRNA or scrambled siRNA, administered by renal artery injection. Treated animals were evaluated for renal IKK? protein and mRNA expression, blood biochemistry, tissue histopathology, NF-?B/DNA binding activity, and expression of two downstream inflammatory cytokines, neutrophil gelatinase-associated lipocalin (NGAL) and IL-18. A local injection of IKK? siRNA resulted in inhibition of renal IKK? gene expression, NF-?B/DNA binding activity, and expression of NGAL and IL-18. Rats pretreated with IKK? siRNA had significantly less blood urea nitrogen and serum creatinine levels and less renal tubular damage scores. Consequently, our data confirm that targeted silencing of IKK? using siRNA substantially diminishes kidney injury and inflammation following ischemia-reperfusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1522-1466
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F857-63
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Small interfering RNA targeting IKK? prevents renal ischemia-reperfusion injury in rats.
pubmed:affiliation
Dept. of Nephrology, Nanjing First Hospital affiliated to Nanjing Medical Univ., Nanjing 210006, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't