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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1991-6-12
|
| pubmed:abstractText |
An accumulation of L-lysine of about 42 g/l (as L-lysine-HCl) was obtained by cultivating Brevibacterium divaricatum NTU-2 in a medium containing 10.0% glucose, 4.0% (NH4)2 SO4, 0.1% KH2 PO4, 0.04% Mg SO4.7H2 O, 30 ml/l soybean meal hydrolysate, 50 mg/l DL-methionine, 100 micrograms/l d-biotin, 100 micrograms/l thiamine-HCl and 5% CaCO3 at pH 7.0. The yield was about 48.8% based on consumed glucose. The L-lysine accumulated in the broth was recovered and purified by simply using a strong cation-exchange resin, Diaion SK1B. The absorbed L-lysine was eluted with 4% ammonia water, and the average adsorption and desorption rates estimated (by 10 repeated experiments) were found to be 96.1% and 97.5%, respectively. The eluate was concentrated to about 50% by volume and adjusted to pH 4.0 with 8 N HCl. The solution was concentrated again to one half volume and followed by adding three volumes of 95% ethanol. The crude crystals of L-lysine-HCl in purity of 89.5% were obtained by filtration. The recovery was 93.5%. The resulting crude crystals were then dissolved in water, and subjected to decolorization with 0.5% active carbon. The decolorized solution thus obtained was recrystalized by concentration and 95% ethanol addition. After filtration and drying at 80 degrees C for 3 hr, white crystalline L-lysine with purity of 99.2% was finally obtained. The overall recovery of L-lysine was up to 86.7%.
|
| pubmed:language |
chi
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0253-2662
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
201-10
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1990
|
| pubmed:articleTitle |
[Lysine production by Brevibacterium divaricatum NTU-2 and its recovery from the fermentation broth].
|
| pubmed:affiliation |
Department of Agricultural Chemistry, National Taiwan University, Taipei, R.O.C.
|
| pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
|