Source:http://linkedlifedata.com/resource/pubmed/id/21278291
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2011-3-14
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pubmed:abstractText |
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1098-5530
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1771-6
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pubmed:dateRevised |
2011-10-3
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pubmed:meshHeading |
pubmed-meshheading:21278291-Animals,
pubmed-meshheading:21278291-Cattle,
pubmed-meshheading:21278291-Cattle Diseases,
pubmed-meshheading:21278291-Chromosomes, Bacterial,
pubmed-meshheading:21278291-DNA, Bacterial,
pubmed-meshheading:21278291-DNA Transposable Elements,
pubmed-meshheading:21278291-Escherichia coli O157,
pubmed-meshheading:21278291-Genetic Fitness,
pubmed-meshheading:21278291-Genotype,
pubmed-meshheading:21278291-Mutagenesis, Insertional,
pubmed-meshheading:21278291-Plasmids
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pubmed:year |
2011
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pubmed:articleTitle |
Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
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pubmed:affiliation |
Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Bush Farm Road, Roslin, Midlothian EH25 9RG, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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