|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1978-12-20
|
|
pubmed:abstractText |
Using in vitro translation of sucrose-gradient fractionated cytoplasmic mRNA from SV40-infected cells, we have shown that a deletion in the region mapping between 0.54--0.59 reduced the size of mRNA for small-t but not the size of mRNA for large-T. Mutants with a deletion in this region were shown to produce in vivo either shortened small-t or no small-t, and normal large-T. Similarly, in vitro translation of poly(A)+cytoplasmic RNA from cells infected with these mutants gave the same results. On the other hand in vitro translation of poly(A)+nuclear RNA from the mutants which made no small-t produced a small-t derivative possibly synthesized from a prespliced RNA species. We have also shown that poly(A)+nuclear RNA from mutant dl 2122 produced two small-t related proteins: one of these (MW: 11K) probably represents the product of a "prespliced" RNA, the other (MW: 17K) which is also found in the cytoplasm represents the product of the mutant specific small-t mRNA.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-14001211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-178902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-179059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-191809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-196762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-198579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-203926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-206886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-206891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-208777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-4292331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-4352646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/212714-823012
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3083-99
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:212714-Antigens, Neoplasm,
pubmed-meshheading:212714-Antigens, Viral,
pubmed-meshheading:212714-Cell Line,
pubmed-meshheading:212714-Cell Nucleus,
pubmed-meshheading:212714-Chromosome Deletion,
pubmed-meshheading:212714-Genes, Viral,
pubmed-meshheading:212714-Molecular Weight,
pubmed-meshheading:212714-Poly A,
pubmed-meshheading:212714-Protein Biosynthesis,
pubmed-meshheading:212714-RNA, Messenger,
pubmed-meshheading:212714-RNA, Viral,
pubmed-meshheading:212714-Simian virus 40
|
|
pubmed:year |
1978
|
|
pubmed:articleTitle |
Characterization of two SV40 early mRNAs and evidence for a nuclear "prespliced" RNA species.
|
|
pubmed:publicationType |
Journal Article
|
