Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-21
pubmed:abstractText
Ischemic preconditioning of remote organs (RIPC) reduces liver ischemia/reperfusion (IR) injury in the rabbit and rat. Mice are the only species available with a large number of transgenic strains. This study describes development and validation of a mouse model of hindlimb RIPC that attenuates liver IR injury. Mice were allocated to 4 groups: (1) Sham surgery; (2) RIPC: 6 cycles of 4 × 4 minutes ischemia/reperfusion of hindlimb; (3) IR: 40 minutes lobar (70%) hepatic ischemia and 2 hours reperfusion; (4) RIPC+IR: RIPC followed by IR group procedures. Plasma liver aminotransferases and hepatic histopathological and transmission electron microscopy studies were performed at the end of the experiment. Hepatic microcirculatory blood flow was measured throughout the experiment. Postoperative complications and animal survival were evaluated. Hindlimb RIPC using a tourniquet resulted in limb paralysis. Hindlimb RIPC using direct clamping of the femoral vessels showed no side effects. Compared to liver IR alone, RIPC+IR reduced plasma aminotransferases (P < 0.05) and histopathological and ultrastructural features of injury. Hepatic microcirculatory blood flow was preserved in the RIPC+IR compared to IR group (P < 0.05). There was no mortality in any of the groups. By demonstrating a consistent improvement in these features of liver IR injury with antecedent hindlimb RIPC and by minimizing experimental confounding variables, we validated this mouse model. In conclusion, we describe a validated mouse model of hindlimb RIPC that reduces liver IR injury. With the availability of transgenic mice strains, this model should prove useful in unraveling the mechanisms of protection of hindlimb RIPC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1527-6473
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 American Association for the Study of Liver Diseases.
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
70-82
pubmed:meshHeading
pubmed-meshheading:21254347-Alanine Transaminase, pubmed-meshheading:21254347-Animals, pubmed-meshheading:21254347-Aspartate Aminotransferases, pubmed-meshheading:21254347-Biological Markers, pubmed-meshheading:21254347-Constriction, pubmed-meshheading:21254347-Disease Models, Animal, pubmed-meshheading:21254347-Hindlimb, pubmed-meshheading:21254347-Ischemic Preconditioning, pubmed-meshheading:21254347-Laser-Doppler Flowmetry, pubmed-meshheading:21254347-Liver, pubmed-meshheading:21254347-Liver Circulation, pubmed-meshheading:21254347-Male, pubmed-meshheading:21254347-Mice, pubmed-meshheading:21254347-Mice, Inbred C57BL, pubmed-meshheading:21254347-Microcirculation, pubmed-meshheading:21254347-Microscopy, Electron, Transmission, pubmed-meshheading:21254347-Muscle, Skeletal, pubmed-meshheading:21254347-Paralysis, pubmed-meshheading:21254347-Reperfusion Injury, pubmed-meshheading:21254347-Reproducibility of Results, pubmed-meshheading:21254347-Time Factors, pubmed-meshheading:21254347-Tourniquets
pubmed:year
2011
pubmed:articleTitle
Effect of remote ischemic preconditioning on liver ischemia/reperfusion injury using a new mouse model.
pubmed:affiliation
Division of Surgery and Interventional Science, University College London, United Kingdom.
pubmed:publicationType
Journal Article