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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-2-4
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pubmed:abstractText |
Type-specific carbohydrate antigen of the serotype i "Streptococcus milleri" was extracted with trichloroacetic acid from purified cell walls of the type strain K39K. The extracts were then purified by chromatography on DEAE-Sephadex A-25 and Sephadex G-100 columns. The purified serotype i carbohydrate antigen produced a single precipitin band against its homologous type-specific antiserum, which fused with the band produced by the autoclaved extract of the type strain cells. The serotype i antigen was a polysaccharide composed of rhamnose, galactose and glucose in a molar ratio of 1.6:6.8:1.0. The quantitative precipitin inhibition test with various haptenic sugars showed that galactose as well as lactose produced the greatest inhibition, which suggested that a galactose in terminal beta linkage is the immunodeterminant of the serotype i-specific antigen. Galactose was detected in the autoclaved extracts from cells of all the 15 serotype i strains tested.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0934-8840
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
40-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2124490-Antigens, Bacterial,
pubmed-meshheading:2124490-Carbohydrates,
pubmed-meshheading:2124490-Cell Wall,
pubmed-meshheading:2124490-Chromatography, Ion Exchange,
pubmed-meshheading:2124490-Humans,
pubmed-meshheading:2124490-Immune Sera,
pubmed-meshheading:2124490-Immunodiffusion,
pubmed-meshheading:2124490-Immunoelectrophoresis,
pubmed-meshheading:2124490-Streptococcus
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pubmed:year |
1990
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pubmed:articleTitle |
Immunochemical characterization of type i carbohydrate antigen of "Streptococcus milleri" (Streptococcus anginosus).
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pubmed:affiliation |
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
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pubmed:publicationType |
Journal Article
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