Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-2-4
pubmed:abstractText
Type-specific carbohydrate antigen of the serotype i "Streptococcus milleri" was extracted with trichloroacetic acid from purified cell walls of the type strain K39K. The extracts were then purified by chromatography on DEAE-Sephadex A-25 and Sephadex G-100 columns. The purified serotype i carbohydrate antigen produced a single precipitin band against its homologous type-specific antiserum, which fused with the band produced by the autoclaved extract of the type strain cells. The serotype i antigen was a polysaccharide composed of rhamnose, galactose and glucose in a molar ratio of 1.6:6.8:1.0. The quantitative precipitin inhibition test with various haptenic sugars showed that galactose as well as lactose produced the greatest inhibition, which suggested that a galactose in terminal beta linkage is the immunodeterminant of the serotype i-specific antigen. Galactose was detected in the autoclaved extracts from cells of all the 15 serotype i strains tested.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0934-8840
pubmed:author
pubmed:issnType
Print
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
40-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Immunochemical characterization of type i carbohydrate antigen of "Streptococcus milleri" (Streptococcus anginosus).
pubmed:affiliation
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
pubmed:publicationType
Journal Article