Source:http://linkedlifedata.com/resource/pubmed/id/21236233
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
2011-2-28
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| pubmed:abstractText |
Helicobacter pylori is the leading cause of gastritis, peptic ulcer disease and gastric adenocarcinoma and lymphoma in humans. Due to the decreasing efficacy of anti-H. pylori antibiotic therapy in clinical practice, there is renewed interest in the development of anti-H. pylori vaccines. In this study an in silico-based approach was utilized to develop a multi-epitope DNA-prime/peptide-boost immunization strategy using informatics tools. The efficacy of this construct was then assessed as a therapeutic vaccine in a mouse model of gastric cancer induced by chronic H. pylori infection. The multi-epitope vaccine administered intranasally induced a broad immune response as determined by interferon-gamma production in ELISpot assays. This was associated with a significant reduction in H. pylori colonization compared with mice immunized with the same vaccine intramuscularly, given an empty plasmid, or given a whole H. pylori lysate intranasally as the immunogen. Total scores of gastric histological changes were not significantly different among the 4 experimental groups. These results suggest that further development of an epitope-based mucosal vaccine may be beneficial in eradicating H. pylori and reducing the burden of the associated gastric diseases in humans.
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| pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/R01 CA111533-02,
http://linkedlifedata.com/resource/pubmed/grant/R01CA111533,
http://linkedlifedata.com/resource/pubmed/grant/R43 AI065036-01,
http://linkedlifedata.com/resource/pubmed/grant/R43AI065036,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI082642-01,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI082642-02,
http://linkedlifedata.com/resource/pubmed/grant/U19AI082642
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1873-2518
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
|
| pubmed:day |
3
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2085-91
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| pubmed:dateRevised |
2011-9-26
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| pubmed:meshHeading |
pubmed-meshheading:21236233-Amino Acid Sequence,
pubmed-meshheading:21236233-Animals,
pubmed-meshheading:21236233-Bacterial Vaccines,
pubmed-meshheading:21236233-Disease Models, Animal,
pubmed-meshheading:21236233-Enzyme-Linked Immunospot Assay,
pubmed-meshheading:21236233-Epitopes, T-Lymphocyte,
pubmed-meshheading:21236233-Female,
pubmed-meshheading:21236233-Helicobacter Infections,
pubmed-meshheading:21236233-Helicobacter pylori,
pubmed-meshheading:21236233-Immunity, Cellular,
pubmed-meshheading:21236233-Immunization, Secondary,
pubmed-meshheading:21236233-Interferon-gamma,
pubmed-meshheading:21236233-Male,
pubmed-meshheading:21236233-Mice,
pubmed-meshheading:21236233-Mice, Inbred C57BL,
pubmed-meshheading:21236233-Molecular Sequence Data,
pubmed-meshheading:21236233-Stomach,
pubmed-meshheading:21236233-Stomach Neoplasms,
pubmed-meshheading:21236233-Vaccines, DNA
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| pubmed:year |
2011
|
| pubmed:articleTitle |
HelicoVax: epitope-based therapeutic Helicobacter pylori vaccination in a mouse model.
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| pubmed:affiliation |
Department of Medicine, Division of Gastroenterology, Rhode Island Hospital & Warren Alpert Medical School of Brown University, Providence, RI 02903, USA. Steven Moss@Brown.edu
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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