Linked Life Data

21221922

Source:http://linkedlifedata.com/resource/pubmed/id/21221922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-1-11
pubmed:abstractText
In this study, a standard strain of HSV-1 (strain SM(44)) was used to investigate the antiviral activity of the recombinant Cyanovirin-N (CV-N) against Herpes simplex virus type 1 (HSV-1) in vitro and in vivo. Cytopathic effect (CPE) and MTT assays were used to evaluate the effect of CV-N on HSV-1 in Vero cells. The number of copies of HSV-DNA was detected by real-time fluorescence quantitative PCR (FQ-PCR). The results showed that CV-N had a low cytotoxicity on Vero cells with a CC(50) of 359.03 ± 0.56 ?g/mL, and that it could not directly inactivate HSV-1 infectivity. CV-N not only reduced the CPE of HSV-1 when added before or after viral infection, with a 50% inhibitory concentration (IC(50)) with 2.26 and 30.16 ?g/mL respectively, but it also decreased the copies of HSV-1 DNA in infected host cells. The encephalitis model for HSV-1 infection was conducted in Kunming mice, and treated with three dosages of CV-N (0.5, 5 & 10 mg/kg) which was administered intraperitoneally at 2h, 3d, 5d, 7d post infection. The duration for the appearance of symptoms of encephalitis and the survival days were recorded and brain tissue samples were obtained for pathological examination (HE staining). Compared with the untreated control group, in the 5mg/kg CV-N and 10mg/kg CV-N treated groups, the mice suffered light symptoms and the number of survival days were more than 9 d and 14 d respectively. HE staining also showed that in 5mg/kg CV-N and 10mg/kg CV-N treated groups, the brain cells did not show visible changes, except for a slight inflammation. Our results demonstrated that CV-N has pronounced antiviral activity against HSV-1 both in vitro and in vivo, and it would be a promising new candidate for anti-HSV therapeutics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1995-820X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
432-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:21221922-Animals, pubmed-meshheading:21221922-Antiviral Agents, pubmed-meshheading:21221922-Bacterial Proteins, pubmed-meshheading:21221922-Biological Agents, pubmed-meshheading:21221922-Carrier Proteins, pubmed-meshheading:21221922-Cell Survival, pubmed-meshheading:21221922-Cercopithecus aethiops, pubmed-meshheading:21221922-Cytopathogenic Effect, Viral, pubmed-meshheading:21221922-DNA, Viral, pubmed-meshheading:21221922-Disease Models, Animal, pubmed-meshheading:21221922-Encephalitis, Viral, pubmed-meshheading:21221922-Female, pubmed-meshheading:21221922-Herpes Simplex, pubmed-meshheading:21221922-Herpesvirus 1, Human, pubmed-meshheading:21221922-Male, pubmed-meshheading:21221922-Mice, pubmed-meshheading:21221922-Recombinant Proteins, pubmed-meshheading:21221922-Survival Analysis, pubmed-meshheading:21221922-Tetrazolium Salts, pubmed-meshheading:21221922-Thiazoles, pubmed-meshheading:21221922-Vero Cells
pubmed:year
2010
pubmed:articleTitle
Antiviral activity of recombinant cyanovirin-N against HSV-1.
pubmed:affiliation
Department of Medical Microbiology, Medical College, Qingdao University, Qingdao 266071, China. yuhong0532@126.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't