Source:http://linkedlifedata.com/resource/pubmed/id/21205187
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2011-3-28
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pubmed:abstractText |
Phospholipase D? (PLD?) is involved in plant response to salt stress, but the mechanisms remain unclear. We investigated rice PLD? (OsPLD?) localization and its effect on tonoplast (TP) and plasma membrane (PM) H(+)-ATPase activity and transcription in response to NaCl. When rice suspension-cultured cells were treated with 100 mM NaCl, PLD? activity in cell extracts showed a transient activation with a threefold increase at 1 h. The amount of OsPLD? protein decreased slightly in the cytosolic fractions, whereas it increased significantly in the TP after NaCl treatment. OsPLD?1 knockdown cells were developed using RNA interference (RNAi) methods. The increase in TP and PM H(+)-ATPase activity induced by NaCl was significantly inhibited in OsPLD?1-RNAi cells. Knockdown of OsPLD?1 prevented the NaCl-induced increase in the transcript level of OsVHA-A (encodes TP H(+)-ATPase) and OSA2 (encodes PM H(+)-ATPase), as well as OsNHX1 (encodes TP Na(+) /H(+) antiporter). The cells died more in OsPLD?1-RNAi mutant than in wild type when they were treated with NaCl. These results suggest that OsPLD? is involved in salt tolerance in rice through the mediation of H(+)-ATPase activity and transcription.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proton-Translocating ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Suspensions,
http://linkedlifedata.com/resource/pubmed/chemical/calcium-dependent protein kinase...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1744-7909
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pubmed:author | |
pubmed:copyrightInfo |
© 2011 Institute of Botany, Chinese Academy of Sciences.
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pubmed:issnType |
Electronic
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-99
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pubmed:meshHeading |
pubmed-meshheading:21205187-Cell Membrane,
pubmed-meshheading:21205187-Cell Survival,
pubmed-meshheading:21205187-Cells, Cultured,
pubmed-meshheading:21205187-Gene Expression Regulation, Plant,
pubmed-meshheading:21205187-Gene Knockdown Techniques,
pubmed-meshheading:21205187-Mutation,
pubmed-meshheading:21205187-Oryza sativa,
pubmed-meshheading:21205187-Phospholipase D,
pubmed-meshheading:21205187-Protein Kinases,
pubmed-meshheading:21205187-Protein Transport,
pubmed-meshheading:21205187-Proton-Translocating ATPases,
pubmed-meshheading:21205187-RNA Interference,
pubmed-meshheading:21205187-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21205187-Salt-Tolerance,
pubmed-meshheading:21205187-Sodium Chloride,
pubmed-meshheading:21205187-Sodium-Hydrogen Antiporter,
pubmed-meshheading:21205187-Stress, Physiological,
pubmed-meshheading:21205187-Suspensions,
pubmed-meshheading:21205187-Transcription, Genetic
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pubmed:year |
2011
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pubmed:articleTitle |
Rice phospholipase D? is involved in salt tolerance by the mediation of H(+)-ATPase activity and transcription.
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pubmed:affiliation |
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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