Source:http://linkedlifedata.com/resource/pubmed/id/21191401
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2011-3-16
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| pubmed:abstractText |
CD160 is expressed by human and mouse natural killer (NK) cells and other cytotoxic lymphocyte subpopulations. CD160 is mostly expressed as a trimeric 83 kDa glycosylphosphatidylinositol (GPI)-anchored activating NK receptor, cleaved upon IL-15 stimulation in a secreted trimeric soluble form (sCD160) that binds to major histocompatibility complex (MHC) class I molecules, while a transmembrane isoform appears. sCD160 exhibits immunoregulatory function as it inhibits CD8(+) T-lymphocyte cytotoxic activity. We show that human mast cells (MCs) express CD160. In human and mouse skin, resident MCs expressed CD160, whereas in C57BL/6-Kit(W-sh/W-sh) mice, CD160(+) cells were only identified at the site of reconstitution with syngeneic cultured MCs. In the human mast cell line, HMC-1, we only identified the transcripts of the GPI-anchored CD160 isoform. Furthermore, CD160 was identified in HMC-1 and mouse MC supernatants, suggesting that MCs release sCD160. Supporting this hypothesis, HMC-1 express the GPI-specific phospholipase D variant 2 involved in the NK lymphocyte membrane cleavage of CD160, and morphological studies highlighted a relative loss of CD160 expression in inflammatory skin sites, where MC degranulation is expected to occur. We also demonstrated an inhibition of T-cell cytotoxicity by HMC-1 supernatant that was partially reversed by anti-CD160 mAb. In conclusion, sCD160, produced by MCs, may have a role in T-cell-MC interactions in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/CD160 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cd160 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1523-1747
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
131
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
916-24
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| pubmed:meshHeading |
pubmed-meshheading:21191401-Animals,
pubmed-meshheading:21191401-Antibodies, Monoclonal,
pubmed-meshheading:21191401-Antigens, CD,
pubmed-meshheading:21191401-COS Cells,
pubmed-meshheading:21191401-Cell Communication,
pubmed-meshheading:21191401-Cercopithecus aethiops,
pubmed-meshheading:21191401-Culture Media, Conditioned,
pubmed-meshheading:21191401-Dermatitis,
pubmed-meshheading:21191401-Dermis,
pubmed-meshheading:21191401-Female,
pubmed-meshheading:21191401-GPI-Linked Proteins,
pubmed-meshheading:21191401-Gene Expression,
pubmed-meshheading:21191401-Humans,
pubmed-meshheading:21191401-Jurkat Cells,
pubmed-meshheading:21191401-Killer Cells, Natural,
pubmed-meshheading:21191401-Mast Cells,
pubmed-meshheading:21191401-Mice,
pubmed-meshheading:21191401-Mice, Inbred C57BL,
pubmed-meshheading:21191401-Mice, Nude,
pubmed-meshheading:21191401-RNA, Messenger,
pubmed-meshheading:21191401-Receptors, Immunologic,
pubmed-meshheading:21191401-Solubility
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| pubmed:year |
2011
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| pubmed:articleTitle |
Human and mouse mast cells express and secrete the GPI-anchored isoform of CD160.
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| pubmed:affiliation |
AP-HP, Groupe Hospitalier Henri Mondor-Albert Chenevier, Department of Pathology, and Université Paris 12, Faculté de Médecine, Créteil, France. nicolas.ortonne@hmn.aphp.fr
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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