Linked Life Data

21184857

Source:http://linkedlifedata.com/resource/pubmed/id/21184857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-1-31
pubmed:abstractText
Virus replicon particles (VRP) are genetically engineered infectious virions incapable of generating progeny virus due to partial or complete deletion of at least one structural gene. VRP fulfil the criteria of a safe vaccine and gene delivery system. With VRP derived from classical swine fever virus (CSF-VRP), a single intradermal vaccination protects from disease. Spreading of the challenge virus in the host is however not completely abolished. Parameters that are critical for immunogenicity of CSF-VRP are not well characterized. Considering the importance of type I interferon (IFN-?/?) to immune defence development, we generated IFN-?/?-inducing VRP to determine how this would influence vaccine efficacy. We also evaluated the effect of co-expressing granulocyte macrophage colony-stimulating factor (GM-CSF) in the vaccine context. The VRP were capable of long-term replication in cell culture despite the presence of IFN-?/?. In vivo, RNA replication was essential for the induction of an immune response. IFN-?/?-inducing and GM-CSF-expressing CSF-VRP were similar to unmodified VRP in terms of antibody and peripheral T-cell responses, and in reducing the blood levels of challenge virus RNA. Importantly, the IFN-?/?-inducing VRP did show increased efficacy over the unmodified VRP in terms of B-cell and T-cell responses, when tested with secondary immune responses by in vitro restimulation assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-2518
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
4
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1491-503
pubmed:meshHeading
pubmed-meshheading:21184857-Animals, pubmed-meshheading:21184857-Antibodies, Neutralizing, pubmed-meshheading:21184857-Antibodies, Viral, pubmed-meshheading:21184857-Antibody Formation, pubmed-meshheading:21184857-Cell Line, pubmed-meshheading:21184857-Classical Swine Fever, pubmed-meshheading:21184857-Classical swine fever virus, pubmed-meshheading:21184857-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:21184857-Interferon-alpha, pubmed-meshheading:21184857-Interferon-beta, pubmed-meshheading:21184857-RNA, Viral, pubmed-meshheading:21184857-Replicon, pubmed-meshheading:21184857-Swine, pubmed-meshheading:21184857-T-Lymphocytes, pubmed-meshheading:21184857-Viral Envelope Proteins, pubmed-meshheading:21184857-Viral Vaccines, pubmed-meshheading:21184857-Virus Replication
pubmed:year
2011
pubmed:articleTitle
Immunogenic and replicative properties of classical swine fever virus replicon particles modified to induce IFN-?/? and carry foreign genes.
pubmed:affiliation
Institute of Virology and Immunoprophylaxis (IVI), Sensemattstrasse 293, CH-3147 Mittelhäusern, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't