Linked Life Data

21181092

Source:http://linkedlifedata.com/resource/pubmed/id/21181092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-1-26
pubmed:abstractText
The glutathione S-transferase P1 (GSTP1) protein plays several critical roles in both normal and neoplastic cells, including phase II xenobiotic metabolism, stress responses, signaling and apoptosis. Overexpression of GSTP1 has been observed in many types of cancer, including head and neck squamous cell carcinoma (HNSCC). However, the role of GSTP1 in HNSCC is not well understood. We investigated the role of GSTP1 in two HNSCC cell lines, HSC3 and SAS. Silencing of GSTP1 revealed that cancer cell proliferation was significantly decreased in both cell lines. In addition, the frequency of apoptotic cells increased following si-GSTP1 transfection of HSC3 and SAS cell lines. Growing evidence suggests that microRNAs (miRNAs) negatively regulate gene expression and can function as oncogenes or tumor suppressors in human cancer. Based on the results of web-based searches, miR-133? is a candidate miRNA targeting GSTP1. Down-regulation of miR-133? has been reported in many types of human cancer, including HNSCC. Transient transfection of miR-133? repressed the expression of GSTP1 at both the mRNA and protein levels. The signal from a luciferase reporter was significantly decreased at one miR-133? target site at the 3'UTR of GSTP1, suggesting that miR-133? directly regulates GSTP1. Our data indicate that GSTP1 may have an oncogenic function and may be regulated by miR-133?, a tumor suppressive miRNA in HNSCC. The identification of a novel oncogenic pathway could provide new insights into potential mechanisms of HNSCC carcinogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1791-244X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345-52
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Glutathione S-transferase P1 (GSTP1) suppresses cell apoptosis and its regulation by miR-133? in head and neck squamous cell carcinoma (HNSCC).
pubmed:affiliation
Department of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't