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Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1990-9-27
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pubmed:abstractText |
The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
16
|
pubmed:volume |
170
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1021-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2117923-Amino Acid Sequence,
pubmed-meshheading:2117923-Base Sequence,
pubmed-meshheading:2117923-DNA, Viral,
pubmed-meshheading:2117923-Hepatitis, Viral, Human,
pubmed-meshheading:2117923-Hepatitis C,
pubmed-meshheading:2117923-Humans,
pubmed-meshheading:2117923-Molecular Sequence Data,
pubmed-meshheading:2117923-Polymerase Chain Reaction,
pubmed-meshheading:2117923-Sequence Homology, Nucleic Acid
|
pubmed:year |
1990
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pubmed:articleTitle |
There are two major types of hepatitis C virus in Japan.
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pubmed:affiliation |
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|