Linked Life Data

21174420

Source:http://linkedlifedata.com/resource/pubmed/id/21174420

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-14
pubmed:abstractText
Among the numerous chemosensors available for diphosphate (P(2)O(7)(4-), PPi) and nucleoside triphosphates (NTPs), only a few can distinguish between PPi and NTPs. Hence, very few bioanalytical applications based on such selective chemosensors have been realized. We have developed a new fluorescence sensing system for distinction between PPi and NTPs based on the combination of two sensors, a binuclear Zn(II) complex (1·2Zn) and boronic acid (BA), in which one chemosensor (1·2Zn) shows signal changes depending on the PPi (or NTP) concentration, and the other (BA) blocks the signal change caused by NTPs; this system enables the distinction of PPi from NTPs and is sensitive to nanomolar concentrations of PPi. The new sensing system has been successfully used for the direct quantification of RNA polymerase activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1520-6904
pubmed:author
pubmed:issnType
Electronic
pubmed:day
21
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-23
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
A highly selective and sensitive fluorescence sensing system for distinction between diphosphate and nucleoside triphosphates.
pubmed:affiliation
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't