Source:http://linkedlifedata.com/resource/pubmed/id/21165790
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2011-2-2
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| pubmed:abstractText |
Human immunodeficiency virus type 1 (HIV-1) nef undergoes adaptive evolution in the central nervous system (CNS), reflecting altered requirements for HIV-1 replication in macrophages/microglia and brain-specific immune selection pressures. The role of Nef in HIV-1 neurotropism and pathogenesis of HIV-associated dementia (HAD) is unclear. In this study, we characterized 82 nef alleles cloned from brain, cerebral spinal fluid, spinal cord, and blood/lymphoid tissue-derived HIV-1 isolates from seven subjects with HAD. CNS isolate-derived nef alleles were genetically compartmentalized and had reduced sequence diversity compared to those from lymphoid tissue isolates. Defective nef alleles predominated in a brain-derived isolate from one of the seven subjects (MACS2-br). The ability of Nef to down-modulate CD4 and MHC class 1 (MHC-1) was generally conserved among nef alleles from both CNS and lymphoid tissues. However, the potency of CD4 and MHC-1 down-modulation was variable, which was associated with sequence alterations known to influence these Nef functions. These results suggest that CD4 and MHC-1 down-modulations are highly conserved functions among nef alleles from CNS- and lymphoid tissue-derived HIV-1 isolates that may contribute to viral replication and escape from immune surveillance in the CNS.
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| pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/MH083588,
http://linkedlifedata.com/resource/pubmed/grant/R01 MH083588-10A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 MH083588-11,
http://linkedlifedata.com/resource/pubmed/grant/R01 MH083588-12,
http://linkedlifedata.com/resource/pubmed/grant/R01 MH083588-14
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1538-2443
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
17
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
82-91
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| pubmed:dateRevised |
2011-8-1
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| pubmed:meshHeading |
pubmed-meshheading:21165790-AIDS Dementia Complex,
pubmed-meshheading:21165790-Alleles,
pubmed-meshheading:21165790-Amino Acid Sequence,
pubmed-meshheading:21165790-Antigens, CD4,
pubmed-meshheading:21165790-Brain,
pubmed-meshheading:21165790-Cell Line,
pubmed-meshheading:21165790-Central Nervous System,
pubmed-meshheading:21165790-Down-Regulation,
pubmed-meshheading:21165790-Genes, MHC Class I,
pubmed-meshheading:21165790-Genes, nef,
pubmed-meshheading:21165790-HIV Infections,
pubmed-meshheading:21165790-HIV-1,
pubmed-meshheading:21165790-Humans,
pubmed-meshheading:21165790-Lymphoid Tissue,
pubmed-meshheading:21165790-Male,
pubmed-meshheading:21165790-Molecular Sequence Data,
pubmed-meshheading:21165790-Phylogeny,
pubmed-meshheading:21165790-Virus Replication,
pubmed-meshheading:21165790-nef Gene Products, Human Immunodeficiency Virus
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| pubmed:year |
2011
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| pubmed:articleTitle |
CD4 and MHC class 1 down-modulation activities of nef alleles from brain- and lymphoid tissue-derived primary HIV-1 isolates.
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| pubmed:affiliation |
Center for Virology, Burnet Institute, 85 Commercial Rd, Melbourne, 3004 VIC, Australia.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|